2007 Fiscal Year Final Research Report Summary
Detection and functional analysis in the genes associated with the development and differentiation of germ cells
| Project/Area Number |
18591766
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
HAYASHI Yutaro Nagoya City University, Graduate School of Medical Sciences, Associate Professor (40238134)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA YOSHIYUKI Nagoya City University, Graduate School of Medical Sciences, Research Associate (60305539)
MARUYAMA Tetsuji Nagoya City University, Graduate School of Medical Sciences, Researcher (50305546)
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| Project Period (FY) |
2006 – 2007
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| Keywords | ES cell / Primordial germ cell / Spermatogenesis / Subtraction / Glucose |
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| Research Abstract |
In a previous study, we established a system for visualizing the development of germ cells from mouse embryonic stem (ES) cells in culture using knock-in ES clones in which visual reporter genes were expressed from the mouse vasa homolog, Mvh. While assessing various culture conditions, we found that germ-cell formation was markedly depressed in low glucose medium. Using a repeated polymerase chain reaction (PCR) subtraction method, we identified genes that were differentially expressed in low versus high glucose media. Three genes that were predominantly expressed in high glucose medium, thioredoxin-interacting protein (Txnip), pituitary tumor-trans forming gene 1 (Pttg), and RuvB-like protein 2 (RuvB12), were further investigated. These genes were also found to be highly expressed in adult and embryonic gonads, and RuvB12 in particular, which encodes an ATP-dependent DNA helicase, was specifically detected in the spermatocytes and spermatids of the adult testis as well as in primordial germ cells. Furthermore, using a green fluorescent protein (GFP) fusion construct, we found that RuvBl2 was expressed in both the nucleus and cytoplasm of testicular germ cells. These findings suggest a possible relationship between glucose metabolism and germ-cell development.
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Author(s)
Havashi, Y, Yasui, T, Koiima, Y, Maruvama, T, Tozawa, K, Kohri, K
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Journal Title
Int J Urol 14
Pages: 161-3
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ventral based dartos flap for the prevention of the urethrocutaneous fistula urethroplasty2007
Author(s)
Hayashi, Y, Kojima, Y, Nakane, A, Kurokawa, S, Tozawa, K, Kohri, K
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Journal Title
Int J Ural 14
Pages: 725-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genes associated with the formation of germ cells from embryonic stem cells in cultures containing different glucose concentrations2006
Author(s)
Mizuno, K, Tokumasu, A, Nakamura, A, Havashi, Y, Kojima, Y, Kohri, K, Noce, T
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Journal Title
Mol Reprod Dev 73
Pages: 437-45
Description
「研究成果報告書概要(欧文)」より
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