2021 Fiscal Year Final Research Report
Improvement of the AID technology through chemical biology approaches
| Project/Area Number |
18H02170
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38060:Applied molecular and cellular biology-related
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
KANEMAKI MASATO 国立遺伝学研究所, 遺伝メカニズム研究系, 教授 (20444507)
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| Co-Investigator(Kenkyū-buntansha) |
林 謙一郎 岡山理科大学, 理学部, 教授 (30289136)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 発現制御 / デグロン / タンパク質分解 |
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| Outline of Final Research Achievements |
The auxin-inducible degron (AID) method allows the degradation of degron-fused proteins in cells by expressing the plant-derived ubiquitin ligase subunit TIR1 by the addition of auxin. The problem with the AID method is that degron-fused proteins are weakly degraded even without auxin.
To overcome this problem, we improved AID. By using a mutant TIR1 and a new ligand, we succeeded in developing AID2, which overcomes the problem. Furthermore, we showed that AID2 can be applied not only to cells but also to living mice. We have published the results of this research in a paper.
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| Free Research Field |
分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
AID2を確立できたことにより、タンパク質分解による発現制御を細胞から個体まで応用できることを示した。タンパク質分解に基づく発現制御は、ごく短時間に標的タンパク質の発現を抑制できるために、標的タンパク質除去直後に起きる影響を観察できる。また、この分解は可逆的であるため、再発現も可能である。本技術は細胞から個体を用いた基礎研究の貢献できるのみならず、創薬における実証実験や疾患モデル動物作成に役立つことが期待される。
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