2020 Fiscal Year Final Research Report
Microsecond resolved single molecule fluorescence measurements triggered by solution mixing
| Project/Area Number |
18H02382
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 一分子蛍光分光法 / 溶液混合装置 |
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| Outline of Final Research Achievements |
We used single molecule fluorescence spectroscopy and revealed the structures and dynamics of biological macromolecules. We developed rapid solution mixing device that can mix two solutions quickly, and enabled the system to be used in combination with the ultrafast single molecule fluorescence detection techniques. We also developed the nano-second region fluorescence correlation spectroscopic method. Using the systems, we investigated the dynamics of various proteins including the wild type of F1-ATPase, the fluorescence intensity fluctuation in the poly-alanine peptide, and the structures of a droplet forming protein, the RGG domain of LAF1.
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| Free Research Field |
タンパク質科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において、揺らぎながら生体中で機能を果たすタンパク質の運動を一分子レベルで観察するための基礎技術を開発した。さらに、開発した技術を用いてさまざまなタンパク質の運動を解明した。このようなデータを積み重ねることで、生体分子の機能を正しく理解し、さらには予想することが可能になると思われる。さらには、薬剤と生体分子の相互作用などの解明に資すると考えられる。
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