mlAOPコンセプトによる化学物質感受性多様性評価と殺虫剤中毒解明に向けた応用

2019 Fiscal Year Annual Research Report

• Project/Area Number: 18H04132
• Research Institution: Hokkaido University
• Principal Investigator: 池中 良徳 北海道大学, 獣医学研究院, 准教授 (40543509)
• Co-Investigator(Kenkyū-buntansha): 星 信彦 神戸大学, 先端融合研究環, 教授 (10209223) ; 川合 佑典 帯広畜産大学, 畜産学部, 助教 (10709546) ; 石塚 真由美 北海道大学, 獣医学研究院, 教授 (50332474) ; 小林 篤史 北海道大学, 獣医学研究院, 准教授 (50431507) ; 久保田 彰 帯広畜産大学, 畜産学部, 准教授 (60432811) ; 水川 葉月 愛媛大学, 農学研究科, 准教授 (60612661) ; 有薗 幸司 熊本県立大学, 環境共生学部, 教授 (70128148) ; 加藤 恵介 東邦大学, 薬学部, 教授 (80276609) ; 宮原 裕一 信州大学, 学術研究院理学系, 教授 (80311330) ; 市川 剛 獨協医科大学, 医学部, 助教 (80438712) ; 岡松 優子 北海道大学, 獣医学研究院, 准教授 (90527178) ; 中山 翔太 北海道大学, 獣医学研究院, 助教 (90647629) ; 平野 哲史 富山大学, 研究推進機構 研究推進総合支援センター, 助教 (70804590)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: mlAOPコンセプト / ネオニコチノイド / 発達神経毒性試験 / 化学物質感受性決定機構

Outline of Annual Research Achievements

本年度は昨年度に引き続き、研究実施計画通り、以下の項目について、実施した。
・mlAOPの基礎データ取得に向けた、げっ歯類およびゼブラフィッシュを用いた各種行動試験の実施：個体レベルでの評価として、様々な系統及び種の動物でオープンフィールドテストや高架式十字迷路等の行動試験を実施した。特に、マウスでは、神経毒性試験の新たな評価法として、２光子顕微鏡を用いた実験および解析を導入し、in vivoにおける化学物質の脳神経系への影響の可視化にチャレンジした。
・組織レベルでの評価のための、イメージング解析を実施：組織レベルでの評価として、昨年度に引き続き、特にイメージング技術を駆使して実施した。特に超高分解能質量顕微鏡（MALDI-FT-ICR-MS 型;Bruker Solarix XR）によるネオニコチノイドおよびその代謝産物の組織分布のイメージング解析を試みた。カテコールアミンに着目した解析では、DPPによる誘導体化により、ドパミンの検出に成功した。一方、現在は再現性の評価や他のニューロトランスミッターへの応用を検討している。
・細胞・分子レベルでの評価：昨年度に引き続き、SH-SY5Y細胞をモデル神経細胞として用い、細胞増殖パラメーターなどの各種項目についてデータを取得した。また、マイクロアレイを実施し、投与により変動する遺伝子群を明らかにした。また、標的分子のリン酸化状態についても解析を実施した。
・ヒト尿試料を用いたバイオモニタリング：昨年度に引き続き、ボランティアを募り、一般市民延べ約1000人から尿試料の採取を実施し、尿中のネオニコチノイド濃度を測定した。また、有機野菜を食べてもらう事による尿中ネオニコチノイド低減効果についても明らかにした。

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

当初計画通り、mlAOPの基礎データ取得のための基盤データを取得するための基盤設備を整えることができ、データも順調に取得出来ている。
２光子顕微鏡を用いた、化学物質に対する新たな神経毒性試験についても、新たな検討を始めており、当初の計画以上に進展している。

Strategy for Future Research Activity

mlAOPの基盤データ取得のため、本年度に引き続き
１：げっ歯類およびゼブラフィッシュを用いた各種行動試験、２：組織レベルでの評価のための、イメージング解析、３：細胞・分子レベルでの評価、４：ヒト尿試料を用いたバイオモニタリングを実施する予定である。
特に１：in vivo実験では、今年度に引き続き、２光子顕微鏡を用いた、ネオニコチノイド投与時の脳ライブイメージングデータを取得予定である。また、２：組織レベルの評価では、誘導体化法を用いることにより、組織イメージングの高感度化を目指す。

Research Products

• [Int'l Joint Research] North-West University/Johannesburg University(南アフリカ)
  • Country Name: SOUTH AFRICA
  • Counterpart Institution: North-West University/Johannesburg University
• [Int'l Joint Research] カセサート大学(タイ)
  • Country Name: THAILAND
  • Counterpart Institution: カセサート大学
• [Int'l Joint Research] モンゴル国立大学/モンゴル獣医生命大学(モンゴル)
  • Country Name: MONGOLIA
  • Counterpart Institution: モンゴル国立大学/モンゴル獣医生命大学
• [Int'l Joint Research] KNUST(ガーナ)
  • Country Name: GHANA
  • Counterpart Institution: KNUST
• [Journal Article] Dichlorodiphenyltrichloroethane (DDT) levels in rat livers collected from a malaria vector control region (2019)
  • Author(s): MOTOHIRA Kodai、IKENAKA Yoshinori、YOHANNES Yared Beyene、NAKAYAMA Shouta M. M.、WEPENER Victor、SMIT Nico J.、VAN VUREN Johan H. J.、SOUSA Ana Catarina、ENUNEKU Alex Ajeh、OGBOMIDA Emmanuel Temiotan、ISHIZUKA Mayumi
  • Journal Title: Journal of Veterinary Medical Science Volume: 81 Pages: 1575～1579
  • DOI: 10.1292/jvms.19-0168
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Growth and neurite stimulating effects of the neonicotinoid pesticide clothianidin on human neuroblastoma SH-SY5Y cells (2019)
  • Author(s): Hirano Tetsushi、Minagawa Satsuki、Furusawa Yukihiro、Yunoki Tatsuya、Ikenaka Yoshinori、Yokoyama Toshifumi、Hoshi Nobuhiko、Tabuchi Yoshiaki
  • Journal Title: Toxicology and Applied Pharmacology Volume: 383 Pages: 114777～114777
  • DOI: 10.1016/j.taap.2019.114777
  • Peer Reviewed
• [Journal Article] How toxic is a non-toxic nanomaterial: Behaviour as an indicator of effect in Danio rerio exposed to nanogold (2019)
  • Author(s): Botha Tarryn L.、Brand Sarel J.、Ikenaka Yoshinori、Nakayama Shouta M.M.、Ishizuka Mayumi、Wepener Victor
  • Journal Title: Aquatic Toxicology Volume: 215 Pages: 105287～105287
  • DOI: 10.1016/j.aquatox.2019.105287
  • Peer Reviewed
• [Journal Article] Tissue distribution and characterization of feline cytochrome P450 genes related to polychlorinated biphenyl exposure (2019)
  • Author(s): Khidkhan Kraisiri、Mizukawa Hazuki、Ikenaka Yoshinori、Nakayama Shouta M.M.、Nomiyama Kei、Yokoyama Nozomu、Ichii Osamu、Darwish Wageh Sobhy、Takiguchi Mitsuyoshi、Tanabe Shinsuke、Ishizuka Mayumi
  • Journal Title: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Volume: 226 Pages: 108613～108613
  • DOI: 10.1016/j.cbpc.2019.108613
  • Peer Reviewed
• [Journal Article] Effects of the organochlorine p,p’-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes (2019)
  • Author(s): Thompson Lesa A.、Ikenaka Yoshinori、Sobhy Darwish Wageh、Nakayama Shouta M.M.、Mizukawa Hazuki、Ishizuka Mayumi
  • Journal Title: Environmental Toxicology and Pharmacology Volume: 72 Pages: 103249～103249
  • DOI: 10.1016/j.etap.2019.103249
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Bioaccumulation of persistent organic pollutants and their trophic transfer through the food web: Human health risks to the rural communities reliant on fish from South Africa's largest floodplain (2019)
  • Author(s): Volschenk C.M.、Gerber R.、Mkhonto M.T.、Ikenaka Y.、Yohannes Y.B.、Nakayama S.、Ishizuka M.、van Vuren J.H.J.、Wepener V.、Smit N.J.
  • Journal Title: Science of The Total Environment Volume: 685 Pages: 1116～1126
  • DOI: 10.1016/j.scitotenv.2019.06.144
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Syntheses of 4‐OH and 5‐OH Imidacloprids (2019)
  • Author(s): Takahashi Keisuke、Tsurumi Tatsuya、Inami Moe、Li Zui、Kusakabe Taichi、Kikkawa Shoko、Azumaya Isao、Tominaga Nobuaki、Ikenaka Yoshinori、Arizono Koji、Kato Keisuke
  • Journal Title: ChemistrySelect Volume: 4 Pages: 7343～7345
  • DOI: 10.1002/slct.201901491
  • Peer Reviewed
• [Journal Article] LC-ESI/MS/MS analysis of neonicotinoids in urine of very low birth weight infants at birth (2019)
  • Author(s): Ichikawa Go、Kuribayashi Ryota、Ikenaka Yoshinori、Ichise Takahiro、Nakayama Shouta M. M.、Ishizuka Mayumi、Taira Kumiko、Fujioka Kazutoshi、Sairenchi Toshimi、Kobashi Gen、Bonmatin Jean-Marc、Yoshihara Shigemi
  • Journal Title: PLOS ONE Volume: 14 Pages: 0219208～0219208
  • DOI: 10.1371/journal.pone.0219208
  • Peer Reviewed
• [Journal Article] Effects of PCB exposure on serum thyroid hormone levels in dogs and cats (2019)
  • Author(s): Takaguchi Kohki、Nishikawa Hiroyuki、Mizukawa Hazuki、Tanoue Rumi、Yokoyama Nozomu、Ichii Osamu、Takiguchi Mitsuyoshi、Nakayama Shouta M.M.、Ikenaka Yoshinori、Kunisue Tatsuya、Ishizuka Mayumi、Tanabe Shinsuke、Iwata Hisato、Nomiyama Kei
  • Journal Title: Science of The Total Environment Volume: 688 Pages: 1172～1183
  • DOI: 10.1016/j.scitotenv.2019.06.300
  • Peer Reviewed
• [Journal Article] One year exposure to Cd- and Pb-contaminated soil causes metal accumulation and alteration of global DNA methylation in rats (2019)
  • Author(s): Nakayama Shouta M.M.、Nakata Hokuto、Ikenaka Yoshinori、Yabe John、Oroszlany Balazs、Yohannes Yared B.、Bortey-Sam Nesta、Muzandu Kaampwe、Choongo Kennedy、Kuritani Takeshi、Nakagawa Mitsuhiro、Ishizuka Mayumi
  • Journal Title: Environmental Pollution Volume: 252 Pages: 1267～1276
  • DOI: 10.1016/j.envpol.2019.05.038
  • Peer Reviewed
• [Journal Article] Evidence of impacts from DDT in pelican, cormorant, stork, and egret eggs from KwaZulu-Natal, South Africa (2019)
  • Author(s): Bouwman H.、Yohannes Y.B.、Nakayama S.M.M.、Motohira K.、Ishizuka M.、Humphries M.S.、van der Schyff V.、du Preez M.、Dinkelmann A.、Ikenaka Y.
  • Journal Title: Chemosphere Volume: 225 Pages: 647～658
  • DOI: 10.1016/j.chemosphere.2019.03.043
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Functional and molecular characterization of UDP-glucuronosyltransferase 2 family in cynomolgus macaques (2019)
  • Author(s): Uno Yasuhiro、Takahira Rika、Murayama Norie、Onozeki Shunsuke、Kawamura Shu、Uehara Shotaro、Ikenaka Yoshinori、Ishizuka Mayumi、Ikushiro Shinichi、Yamazaki Hiroshi
  • Journal Title: Biochemical Pharmacology Volume: 163 Pages: 335～344
  • DOI: 10.1016/j.bcp.2019.03.002
  • Peer Reviewed
• [Journal Article] Baseline bio-accumulation concentrations and resulting oxidative stress in Synodontis zambezensis after an acute laboratory exposure to 4,4′-DDT (2019)
  • Author(s): Volschenk C.M.、Ikenaka Y.、Yohannes Y.B.、Nakayama S.M.、Ishizuka M.、Smit E.、van Vuren J.H.J.、Greenfield R.
  • Journal Title: Pesticide Biochemistry and Physiology Volume: 156 Pages: 44～55
  • DOI: 10.1016/j.pestbp.2019.02.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A review: poisoning by anticoagulant rodenticides in non-target animals globally (2019)
  • Author(s): NAKAYAMA Shouta M.M.、MORITA Ayuko、IKENAKA Yoshinori、MIZUKAWA Hazuki、ISHIZUKA Mayumi
  • Journal Title: Journal of Veterinary Medical Science Volume: 81 Pages: 298～313
  • DOI: 10.1292/jvms.17-0717
  • Peer Reviewed
• [Journal Article] Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species (2019)
  • Author(s): Kawai Yusuke K.、Shinya So、Ikenaka Yoshinori、Saengtienchai Aksorn、Kondo Takamitsu、Darwish Wageh Sobhy、Nakayama Shota M.M.、Mizukawa Hazuki、Ishizuka Mayumi
  • Journal Title: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Volume: 217 Pages: 5～14
  • DOI: 10.1016/j.cbpc.2018.11.001
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Species differences in xenobiotic metabolism by phase I and II enzymes of companion animals and wildlife. (2019)
  • Author(s): Mayumi Ishizuka, Yoshinori Ikenaka, Shouta MM Nakayama.ｖ
  • Organizer: The 2nd Joint Meeting of Veterinary Science in East Asia
  • Int'l Joint Research / Invited
• [Presentation] The trend of elevated blood lead levels in Kabwe mining area, Republic of Zambia. (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, John Yabe, Kaampwe Muzandu, Yared B. Yohannes, Haruya Toyomaki, Andrew Kataba, Golden Zyambo, Hazuki Mizukawa, Yoshinori Ikenaka, Russel Dowling, Jack Caravanos, Mayumi Ishizuka.
  • Organizer: SETAC Europe 29th Annual Meeting.
  • Int'l Joint Research
• [Presentation] Cost-effective mitigation of lead toxicity in rat using indigenous plant Moringa Oleifera. (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Andrew Kataba, Haruya Toyomaki, Rio Doya, John Yabe, Kaampwe Muzandu, Golden Zyambo, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka.
  • Organizer: SETAC Europe 29th Annual Meeting.
  • Int'l Joint Research
• [Presentation] 血漿中のステロイドホルモンの一斉分析法の開発とウマ性ホルモンの季節変動の解明 (2019)
  • Author(s): 一瀬貴大、池中良徳、三津橋嵩史、中山翔太、Aksorn Saengtienchai、野見山桂、田上瑠美、石塚真由美
  • Organizer: 第28回環境化学討論会
• [Presentation] 野生ラットを用いたDDT汚染状況の評価、毒性機序の解明 (2019)
  • Author(s): 本平航大、池中良徳、ヤレド ベイェネ、中山翔太、ビクター ベペナー、ニコ スミッツ、ヨハン ファン フューレン、江口哲史、アナ カタリナ ソウサ、石塚真由美
  • Organizer: 第28回環境化学討論会
• [Presentation] LC/MSを用いたイヌ血清中ステロイドホルモンの分析法の確立と副腎疾患モデルを用いた実測 (2019)
  • Author(s): 白尾大樹、池中良徳、一瀬貴大、中山翔太、佐々木東、永田矩之、水川葉月、滝口満善、石塚真由美
  • Organizer: 第28回環境化学討論会
• [Presentation] Rodent tooth as a tool for environmental lead (Pb) assessment (2019)
  • Author(s): Andrew Kataba, Shouta M.M. Nakayama, Hokuto Nakata,Haruya Toyomaki,Yared B. Yohannes,Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] Neonicotinoid Insecticide and High Fat Diet Interactions Potentiate Sidnals of Type 2 Diabetogenesis in a Rodent Model (2019)
  • Author(s): Collins Nimako, Yoshinori Ikenaka, Yuko Okamatsu-Ogura, Atsushi Kobayashi, Shouta M.M. Nakayama, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] DNA methylation in CpG-rich promoter region and Genetic polymorphism of ALAD gene in environmental Lead-Exposed Children from Kabwe, Zambia (2019)
  • Author(s): Yared B. Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, , Kaampwe Muzandu, Andrew Kataba,Yoshinori Ikenaka, Kennedy Choongo, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] Seasonal change of lead contamination levels in wild rat blood and soil (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Golden Zyambo, John Yabe, Kaampwe Muzandu, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] ザンビア共和国カブエ地域における資源採掘に起因した鉛汚染の実態解明と対策の検討 (2019)
  • Author(s): 中田北斗、中山翔太、John Yabe、Kaampwe Muzandu、豊巻治也、Yared Beyene、Andrew Kataba、Golden Zyambo、Kennedy Choongo、池中良徳、石塚真由美
  • Organizer: 第28回環境化学討論会
• [Presentation] Investigation of ameliorative effect of Moringa Oleifera on lead toxicity (2019)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Andrew Kataba, Haruya Toyomaki, Rio Doya, John Yabe, Kaampwe Muzandu, Golden Zyambo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] 摂食プラスチックから海鳥への化学物質の移行の検証 (2019)
  • Author(s): 田中厚資、綿貫豊、高田秀重、石塚真由美、山下麗、水川薫子、水川葉月、池中良徳、中山翔太
  • Organizer: 第28回環境化学討論会
• [Presentation] In Vivo Pharmacokinetic/pharmacodynamic analysis of first generation anticoagulant rodenticide warfarin with Egyptian fruit bats ( Rousettus aegyptiacus ) for risk assessment of Bonin fruit bats Pteropus pselaphon (2019)
  • Author(s): Shouta M.M. Nakayama, Kosuke Manago, Kazuki Takeda, Ayuko Morita, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] Characterization and differentiation of xenobiotic metabolism in mammalian animals through glucuronidation and sulfation profiling (2019)
  • Author(s): Aksorn Saengtienchai, Yoshinori Ikenaka, Shouta M.M. Nakayama, Mayumi Ishizuka
  • Organizer: 第28回環境化学討論会
• [Presentation] ネオニコチノイドの母子間移行の実態と移行メカニズムの解明 (2019)
  • Author(s): 池中良徳、一瀬貴大、ニマコ・コリンズ、市川剛、野見山桂、長谷川浩、中山翔太、星信彦、平久美子、石塚真由美
  • Organizer: 第28回環境化学討論会
• [Presentation] PCBs 曝露によるネコ肝トランスクリプトームへの影響 (2019)
  • Author(s): 水川葉月、Hoa Thanh Ngyuen、岩田久人、野見山桂 、池中良徳、中山翔太、横山望 、市居修 、滝口満喜、Kraisiri Khidkhan、田辺信介、石塚真由美
  • Organizer: 第28回環境化学討論会
• [Presentation] デカブロモジフェニルエーテル（BDE209）の長期曝露がイエネコへ及ぼす影響 (2019)
  • Author(s): 田中啓介、野見山桂、水川葉月、髙口倖暉、田上瑠美、横山望、市居修、滝口満喜、笹岡一慶、中山翔太、池中良徳、石塚真由美、国末達也、田辺信介
  • Organizer: 第28回環境化学討論会
• [Presentation] 鳥類の鉛暴露の実態とその毒性 (2019)
  • Author(s): 石塚真由美、池中良徳、中山翔太
  • Organizer: 希少な鳥を守るには ―野鳥の鳥インフルエンザと鉛汚染対策に向けて― 公開シンポジウム
  • Int'l Joint Research
• [Presentation] Contamination Status of Organohalogen Compounds in Pet Cats, Cat Food and House Dust from Thailand (2019)
  • Author(s): Makoto Shimasaki, Hazuki Mizukawa, Kohki Takaguchi, Aksorn Saengtienchai, Araya Ngamchirttakul, Disdanai Pencharee, Kraisiri Khidkhan, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M.M. Nakayama, Tatsuya Kunisue, Shinsuke Tanabe, Kei Nomiyama
  • Organizer: 第28回環境化学討論会
• [Presentation] Neonicotinoid Insecticide and High Fat Diet Interactions Potentiate Sidnals of Type 2 Diabetogenesis in a Rodent Model (2019)
  • Author(s): Collins Nimako, Yoshinori Ikenaka, Yuko Okamatsu-Ogura, Atsushi Kobayashi, Ryo Yamazaki, Shouta M.M. Nakayama, Mayumi Ishizuka
  • Organizer: SETAC-Australasia 2019
  • Int'l Joint Research
• [Presentation] Investigation of mechanism of rodenticide resistance with closed colonies of rodenticide resistant rats from Tokyo (2019)
  • Author(s): Kazuki Takeda, Yoshinori Ikenaka, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Tsutomu Tanikawa and Mayumi Ishizuka
  • Organizer: 15th International Congress of Toxicology
  • Int'l Joint Research
• [Presentation] Determination of Lead (Pb) exposure on infants living around Pb mining area in Kabwe, Zambia (2019)
  • Author(s): Haruya Toyomaki, John Yabe, Shouta Nakayama, Yared Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Takeshi Kuritani, Mitsuhiro Nakagawa, Mayumi Ishizuka
  • Organizer: the 15th International Congress of Toxicology
  • Int'l Joint Research
• [Presentation] PCR法によるオオワシ(Haliaeetus pelagicus)の孵化前雌雄判定 (2019)
  • Author(s): 中山翔太、平野なぎ沙、境秀文、五島渉、伊藤真輝、池中良徳、石塚 真由美
  • Organizer: 第25回日本野生動物医学会大会（山口）
• [Presentation] 東京都小笠原諸島における殺鼠剤散布のアオウミガメへのリスク評価 (2019)
  • Author(s): 山村快哉、武田一貴、中山翔太、川合佑典、森田鮎子、小松謙之、北山知代、近藤理美、池中良徳、石塚真由美
  • Organizer: 第25回日本野生動物医学会大会（山口）
• [Presentation] 鉛汚染は末梢血中の免疫系細胞を変動させるのか？ (2019)
  • Author(s): 牛根奈々、中山翔太、石塚真由美、羽山伸一
  • Organizer: 第25回日本野生動物医学会大会（山口）
• [Presentation] 体内動態および行動毒性試験から見た動物用医薬品フィプロニルの影響評価 (2019)
  • Author(s): 鈴木智裕、池中良徳、武田一貴、中山翔太、水川葉月、一瀬貴大、佐々木東、笹岡一慶、滝口満喜、石塚真由美
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] LC-MS/MSを用いたイヌ血清中ステロイドホルモンの分析法の確立と内分泌疾患モデルを用いた実測 (2019)
  • Author(s): 白尾 大樹、池中 良徳、一瀬 貴大、中山 翔太、佐々木 東、永田 矩之、
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] 鉛散弾の経口投与によるバルバリーガモの鉛の血中濃度の経時変化および体内分布の解析 (2019)
  • Author(s): 佐藤裕、石井千尋、中山翔太、一瀬貴大、齊藤慶輔、渡邊有希子、小笠原浩平、鳥本亮太、小林篤史、木村亨史、池中良徳、石塚真由美
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] LC-MS/MSを用いたイヌ尿中メタネフリンおよびノルメタネフリン測定 (2019)
  • Author(s): 井上裕美子、佐々木東、池中良徳、一瀬貴大、笹岡一慶、大菅辰幸、中山翔太、森下啓太郎、大田寛、石塚真由美、滝口満喜
  • Organizer: 第162回日本獣医学会学術集会
• [Presentation] Interactions of Imidacloprid with High Fat Diet Potentiate Signals of Type 2 Diabetogenesis in a Rodent Model (2019)
  • Author(s): Collins NIMAKO, Yoshinori IKENAKA, Yuko OKAMATSU-OGURA, Atsushi KOBAYASHI, Shouta M.M. NAKAYAMA and Mayumi ISHIZUKA
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] Rodent tooth as a biomarker for lead (Pb) exposure (2019)
  • Author(s): Andrew Kataba, Shouta M.M. Nakayama, Hokuto Nakata,Haruya Toyomaki,Yared B. Yohannes,Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] G177C Polymorphism and Promoter CpG Methylation of ALAD Gene in Environmental Lead-Exposed Children (2019)
  • Author(s): Yared B. Yohannes, Shouta Nakayama, John Yabe, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Hokuto Nakata,Yoshinori Ikenaka, Kennedy Chongo, Mayumi Ishizuka
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] Current Trends of Blood Lead Levels, Distribution Patterns and Exposure Variations among Household Members in Kabwe, Zambia (2019)
  • Author(s): John Yabe, Shouta MM Nakayama, Hokuto Nakata, Haruya Toyomaki, Yared B Yohannes, Kaampwe Muzandu, Andrew Kataba, Golden Zyambo, Masato Hiwatari, Daiju Narita, Daichi Yamada, Peter Hangoma, Nosiku Sipilanyambe Munyinda, Tiza Mufune, Yoshinori Ikenaka, Kennedy Choongo, Mayumi Ishizuka
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] Comparative metabolism of PCBs by cytochrome P450 in cat and dog (2019)
  • Author(s): Kraisiri KHIDKHAN, Hazuki MIZUKAWA, Makoto SHIMASAKI, Kei NOMIYAMA, Yoshinori IKENAKA, Shouta M.M. NAKAYAMA, Hisato IWATA and Mayumi ISHIZUKA
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] In vivo analysis of rodenticides with Egyptian fruit bats (2019)
  • Author(s): Kosuke MANAGO, Kazuki TAKEDA, Shouta MM NAKAYAMA, Ayuko MORITA, Yoshinori IKENAKA, Mayumi ISHIZUKA
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] Monitoring of Dichloro-diphenyl-trichloroethane Accumulation Levels and Elucidation of its Toxicity in the Field Study (2019)
  • Author(s): Kodai Motohira, Yoshinori Ikenaka, Yared Beyene, Shouta Nakayama, Victor Wepener, Nico Smit, Johan van Vuren, Akifumi Eguchi, Mayumi Ishizuka
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] Application of adverse outcome pathways as arisk assessment tool of chemical stressors in wildlife and humans (2019)
  • Author(s): Victor Wepener, Yoshinori Ikenaka, Suanne Bosch, Avela Mbangatha, Shouta MM Nakayama, Mayumi Ishizuka, Tarryn L Botha
  • Organizer: The 7th Sapporo Summer Symposium for One Health (7th SaSSOH)
  • Int'l Joint Research
• [Presentation] 抗凝血系殺鼠剤が小笠原諸島在来固有種に与える影響の評価 (2019)
  • Author(s): 真砂皓大、武田 一貴、中山 翔太、森田 鮎子、池中 良徳、石塚 真由美
  • Organizer: 第25回 日本環境毒性学会研究発表会
• [Presentation] 単回投与試験によるコウモリの抗凝血系殺鼠剤リスクアセスメントの実施 (2019)
  • Author(s): 真砂皓大、武田 一貴、中山 翔太、森田 鮎子、池中 良徳、石塚 真由美
  • Organizer: 第25回 日本環境毒性学会研究発表会
• [Presentation] Time trend of blood Pb concentration and toxicological effects of Pb gunshots through oral administration in waterfowl’s (2019)
  • Author(s): Hiroshi Sato, Chihiro Ishii, Shouta M. M. Nakayama, Takahiro Ichise, Keisuke Saito, Yukiko Watanabe, Kohei Ogasawara,Ryota Torimoto, Atushi Kobayashi, Takashi Kimura, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第 3 回環境化学会 北海道東北地区部会 & 3rd Chemical Hazard Symposium in 札幌
• [Presentation] 摂食プラスチックから海鳥への添加由来化学物質の移行 (2019)
  • Author(s): 田中厚資, 綿貫豊，高田秀重，石塚真由美, 山下麗, 水川薫子, 水川葉月, 池中良徳, 中山翔太
  • Organizer: 第 3 回環境化学会 北海道東北地区部会 & 3rd Chemical Hazard Symposium in 札幌
• [Presentation] Interactions of Imidacloprid with High Fat Diet Potentiate Signals of Type 2 Diabetogenesis in a Rodent Model (2019)
  • Author(s): Collins NIMAKO, Yoshinori IKENAKA, Yuko OKAMATSU-OGURA, Atsushi KOBAYASHI, Shouta M.M. NAKAYAMA and Mayumi ISHIZUKA
  • Organizer: 第 3 回環境化学会 北海道東北地区部会 & 3rd Chemical Hazard Symposium in 札幌
• [Presentation] Impact of Lead Exposure, Distribution Patterns and Exposure Variations among Household Members in Kabwe, Zambia (2019)
  • Author(s): Yared B. Yohannes, Shouta Nakayama, John Yabe, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Hokuto Nakata,Yoshinori Ikenaka, Kennedy Chongo, Mayumi Ishizuka
  • Organizer: 第 3 回環境化学会 北海道東北地区部会 & 3rd Chemical Hazard Symposium in 札幌
• [Presentation] 室内化学物質汚染の実態解明とリスク評価～ペットはヒトの歩哨動物として用いられるか？～ (2019)
  • Author(s): Hazuki Mizukawa, Kei Nomiyama, Yoshinori Ikenaka, Kraisiri Khidkhan, Shouta MM Nakayama, Mayumi Ishizuka
  • Organizer: 第 3 回環境化学会 北海道東北地区部会 & 3rd Chemical Hazard Symposium in 札幌
• [Presentation] タイのペットネコにおける有機ハロゲン化合物の汚染実態 (2019)
  • Author(s): Makoto Shimasaki, Hazuki Mizukawa, Kohki Takaguchi, Aksorn Saengtienchai, Araya Ngamchirttakul, Disdanai Pencharee, Kraisiri Khidkhan, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M.M. Nakayama, Tatsuya Kunisue, Shinsuke Tanabe, Kei Nomiyama
  • Organizer: 第 3 回環境化学会 北海道東北地区部会 & 3rd Chemical Hazard Symposium in 札幌
• [Presentation] Pharmacokinetic/pharmacodynamic analysis of first-generation anticoagulant rodenticide warfarin with Egyptian fruit bats (Rousettus aegyptiacus) (2019)
  • Author(s): Shouta MM NAKAYAMA, Kosuke MANAGO, Kazuki TAKEDA, Ayuko MORITA, Yoshinori IKENAKA, Mayumi ISHIZUKA
  • Organizer: SETAC North America 40th Annual Meeting
  • Int'l Joint Research
• [Presentation] Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) reveals detailed distribution patterns of Pb in mice organs (2019)
  • Author(s): Shouta M.M. Nakayama, Masao Togao, Yoshinori Ikenaka, Yoshiki Makino, Ayano Kubota, Takehisa Matsukawa, Kazuhito Yokoyama, Takafumi Hirata, Mayumi Ishizuka
  • Organizer: SETAC North America 40th Annual Meeting
  • Int'l Joint Research
• [Presentation] 「基質結合部位からも活性残基からも離れたアミノ酸変異はどのように殺鼠剤抵抗性に寄与するのか？」ドッキングシミュレーション・分子動力学シミュレーションによるビタミンKエポキシド還元酵素の解析 (2019)
  • Author(s): 武田一貴、池中良徳、フルシュデニス、田中和之、中山翔太、谷川力、トリキドハ、シンハオリー、石塚真由美
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] DDTによる異物代謝酵素の遺伝子発現撹乱と健康影響評価 (2019)
  • Author(s): 本平 航大, 池中 良徳, Yared Beyene, 中山 翔太, 江口 哲史, 武田 一貴, Victor Wepener, Nico Smit, Johan Van Vuren, 石塚 真由美
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Tracing Life History of Immature Antarctic Minke Whales: Stable Isotope Oscillation in Baleen Revealed Ontogenetic Diet Shifts and Seasonal Migration (2019)
  • Author(s): Ippei Suzuki, Mayuka Uchida, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M.M. Nakayama, Tsutomu Tamura, Kenji Konishi, Takeharu Bando, Yoko Mitani
  • Organizer: 2019 World Marine Mammal Science Conference
  • Int'l Joint Research
• [Presentation] Sensitivity of turtles to anticoagulant rodenticides: in vivo and in vitro sensitivity evaluation in green sea turtles (Chelonia mydas mydas) in the Ogasawara Islands (2019)
  • Author(s): Shouta MM Nakayama, Yoshiya Yamamura, Kazuki Takeda, Yusuke Kawai, Ayuko Morita, Yoshinori Ikenaka, Noriyuki Komatsu, Chiho Kezuka, Mari Taniguchi, Chiyo Kitayama, Satomi Kondo, Mayumi Ishizuka
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Development of simultaneous analysis method of steroid hormones in blood and measurement of canine adrenal disease (2019)
  • Author(s): Takahiro Ichise，Yoshinori Ikenaka，Diki Shirao， Syouta Nakayama， Mayumi Ishizuka
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Maternal to fetal transfer of systemic pesticide Clothianidin and its metabolites (2019)
  • Author(s): Mai Tamba, Yoshinori Ikenaka, Shuji Ohno, Takahiro Ichise, Shouta M.M. Nakayama, Mayumi Ishizuka and Nobuhiko Hoshi
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Investigation of the toxicity of DDT in wildlife focusing on sex linked gene expression (2019)
  • Author(s): Kodai Motohira, Ikenaka Y,YARED YB, Nakayama SMM , Wepener V, Smit N, Vuren JV, Ishizuka M
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Elucidation of the Effects of Low Dose Exposures to Imidacloprid on Hepatic Lipid Homeostasis in a C57BL/6J Mice Model (2019)
  • Author(s): Collins NIMAKO, Yoshinori IKENAKA, Yuko OKAMATSU-OGURA, Atsushi KOBAYASHI, Ryo YAMAZAKI, Shouta M.M. NAKAYAMA, Mayumi ISHIZUKA
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Pharmacokinetics/dynamics analysis of anticoagulant rodenticides with Egyptian fruit bats (Rousettus aegyptiacus) (2019)
  • Author(s): Kazuki Takeda, Kosuke Manago, Shouta MM Nakayama, Ayuko Morita,Yoshinori Ikenaka, and Mayumi Ishizuka
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Species differences in cytochrome P450-mediated metabolism of polychlorinated biphenyls between cats and dogs (2019)
  • Author(s): Kraisiri KHIDKHAN, Hazuki MIZUKAWA, Makoto SHIMASAKI, Kei NOMIYAMA, Yoshinori IKENAKA, Shouta M.M. NAKAYAMA, Hisato IWATA and Mayumi ISHIZUKA
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Blood Lead Levels and ALAD Promoter Methylation among Environmental Lead-Exposed Children from Kabwe, Zambia (2019)
  • Author(s): YARED B. Yohannes, Nakayama SMM, Yabe J, Toyomaki H, Kataba A, Muzandu K, Nakata H, Ikenaka Y, Choongo K, Ishizuka M
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Effects of environmentally relevant lead (Pb) acute exposure on the locomotion behaviour and molecular subcellular responses in the larvae of Danio rerio (zebrafish). (2019)
  • Author(s): Andrew Kataba,Tarryn Lee Botha,Shota MM Nakayama,Yoshinori Ikenaka, Victor Wepener,Mayumi Ishizuka
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Organ Pb distribution and temporal change of blood Pb concentrations with oral administration of Pb bullets in waterfowls and raptors (2019)
  • Author(s): Hiroshi SATO, Chihiro ISHII, Shouta M. M. NAKAYAMA, Takahiro ICHISE, Keisuke SAITO, Yukiko WATANABE, Kohei OGASAWARA, Ryota TORIMOTO, Atushi KOBAYASHI, Takashi KIMURA, Yoshinori IKENAKA, Mayumi ISHIZUKA
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Screening of metal-related toxicity in residents of Kabwe, Zambia (2019)
  • Author(s): Hokuto Nakata, Shouta MM Nakayama, John Yabe, Kaampwe Muzandu, Haruya Toyomaki, Yared B Yohannes, Andrew Kataba, Golden Zyambo, Yoshinori Ikenaka, Kennedy Choongo, Mayumi Ishizuka
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Seabirds are exposed to chemical additives through plastic ingestion (2019)
  • Author(s): Kosuke Tanaka, Yutaka Watanuki, Hideshige Takada, Yosinori Ikenaka, Shouta Nakayama, Rei Yamashita, Mayumi Ishizuka
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Development of monitoring method for neurotransmission disturbance of nicotinic acetylcholine receptor agonist ~Live imaging of cranial nerve activity using two-photon microscopy ~ (2019)
  • Author(s): Anri Hirai，Shouta Sugio，Yoshinori Ikenaka， Collins Nimako，Shouta M.M. Nakayama, Nobuhiko Hoshi, Hiroaki Wake, Mayumi Ishizuka
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] Evolution of Avian UDP-glucuronosyltransferase Genes in Relation to Feeding Habit (2019)
  • Author(s): Kawai YK, Shinya S, Nakayama SMM, Ikenaka Y, Ishizuka M, Kubota A
  • Organizer: International Symposium on Chemical Hazard in Wildlife
  • Int'l Joint Research
• [Presentation] ヒゲ板中安定同位体比から推定するクロミンククジラ成熟雄の回遊生態 (2019)
  • Author(s): 石川啓史、石塚真由美、池中良徳、中山翔太、坂東武治、田村力、小西健志、大槻真友子、三谷曜子
  • Organizer: 令和２年度日本水産学会春季大会
• [Presentation] Gene Specific Promoter Methylation of Lead Exposure Biomarker Genes in Environmental Lead-Exposed Children from Kabwe, Zambia (2019)
  • Author(s): YARED B. Yohannes, Shouta Nakayama, John Yabe, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Hokuto Nakata, Yoshinori Ikenaka, Kennedy Choongo, Mayumi Ishizuka
  • Organizer: The 2020 SOT Annual Meeting
  • Int'l Joint Research
• [Remarks] 北海道大学大学院獣医学研究院 毒性学教室 ホームページ
  • URL: http://tox.vetmed.hokudai.ac.jp/research/presentations
• [Funded Workshop] International Symposium on Chemical Hazard in Wildlife (2020)