Estanblishment of cell cuture system to reproduce affinty maturaton of antiby

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K04852
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Okayama University
• Principal Investigator: Magari Masaki 岡山大学, ヘルスシステム統合科学研究科, 助教 (50359882)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 抗体の親和性成熟 / 胚中心 / 濾胞樹状細胞 / B細胞

Outline of Final Research Achievements

Affinity maturation is the process to improve antibody affinity for an antigen, which is mediated by somatic hypermutation (SHM) of immunoglobulin (Ig) genes and clonal selection of high-affinity B cells. In this study, we examined whether a novel type of monocytic cell (FDMC) was involved in affinity maturation of antibody. Firstly, we revealed that FDMC induced not only somatic hypermutation at Ig gene but also apoptosis in cultured B cells. We also observed the reduced number of apoptotic cell in Bim-deficient B cells compared with that in wild-type B cells, when B cells were cultured with FDMC. Furthermore, the ability of B cell activation was observed in cultured medium of FDMCs, suggesting that B cell activation was promoted by soluble factor(s) secreted by FDMC.

Free Research Field

免疫学，細胞工学

Academic Significance and Societal Importance of the Research Achievements

抗体の親和性成熟機構は，免疫能力を高めるために重要な免疫反応であることが知られている．そのため，抗体の親和性成熟機構の解明は効果的な免疫能力を誘導できるワクチンの開発や免疫賦活剤の開発につながると考えられる．また，その高い効果から近年注目されている抗体医薬の開発に貢献できる．