2020 Fiscal Year Final Research Report
Construction of bacterial strains capable of degrading persistent chlorinated organophosphorous compounds with high efficiency
| Project/Area Number |
18K05197
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 34030:Green sustainable chemistry and environmental chemistry-related
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| Research Institution | Hakodate National College of Technology (2020)
Nagaoka University of Technology (2018-2019) |
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Principal Investigator |
Abe Katsumasa 函館工業高等専門学校, 物質環境工学科, 准教授 (40509551)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 環境浄化 / 有機リン化合物 / 組換え大腸菌 / バイオレメディエーション / 微生物分解 |
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| Outline of Final Research Achievements |
The persistent chlorinated organophosphate triester, tris(2-chloroethyl) phosphate (TCEP), has been widely used as a flame retardant and a plasticizer. Recently, toxicity of TCEP has been reported and thus raises concern about its harmful effect on organisms including human. In this study, I constructed a novel recombinant E. coli capable of degrading TCEP. The recombinant E. coli degraded TCEP about eight times faster than the wild-type strain, and was also found to efficiently degrade high concentrations of TCEP.
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| Free Research Field |
環境微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
難分解性環境汚染物質であるtris(2-chloroethyl) phosphateの微生物分解システムの開発が望まれているが，現在のTCEP分解菌の分解速度は非常に遅い（低濃度でも4日）.本研究で構築された高機能TCEP分解菌は上記と同じ低濃度のTCEPであれば8時間程度で，その10倍濃度のTCEPであっても2日以内に分解可能であることを示した.本研究成果はTCEP汚染環境の修復などの環境技術開発，および，難分解性環境汚染物質の微生物分解メカニズムの解明において重要な知見であり，その社会的意義や学術的意義は大きい．
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