Evaluation of genetic basis for life history polymorphism in masu salmon: based on genetics related to metabolism

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K05796
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: Yamamoto Toshiaki 日本獣医生命科学大学, 獣医学部, 准教授 (30409255)
• Co-Investigator(Kenkyū-buntansha): 北西 滋 大分大学, 理工学部, 准教授 (90552456)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 遺伝子発現 / スモルト / 生活史多型 / RNA-seq

Outline of Final Research Achievements

In this study, in order to clarify a genetic basis for the life history polymorphism in masu salmon Oncorhynchus masou, I conducted the GRAS-Di analysis and RNA-seq analysis using a next-generation sequence. As a result, some candidate genes in conjunction with the smolting were able to detect. On the other hand, the candidate genes in conjunction with the sexual maturation as the resident form was complicated, and it was suggested that many hereditary domains was related to determine whether individuals are the maturation. Besides, I crossbred using some parents of the different life history and tested gene expression of offspring based on half-sib in each time. As a result, the expression of the candidate genes in conjunction with the smolting showed that it did not express at least until the early stage of life history.

Free Research Field

分子生態学

Academic Significance and Societal Importance of the Research Achievements

生活史多型は様々な動物群においてみられる現象であり、これまでに多くの研究者を魅了してきたが、それら生活史に関連した遺伝子は未だ十分に解明されていない。本研究では、スモルト期に関連しいる遺伝子が限定的にすることが出来たことは、初めての生活史多型に関連した遺伝子の解明につながると考えられることから、非常に学術的な意義は高い。さらには、水産資源としても重要なサケ科魚類では、関連遺伝子が明らかになった場合においては、効率的な資源作成にもつながることが期待されるため、社会的なインパクトも非常に大きいと考えらる。