Analysis of the mechanism of increase in antinuclear antibody levels due to differences in genetic background of mice

2023 Fiscal Year Final Research Report

• Project/Area Number: 18K06027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42040:Laboratory animal science-related
• Research Institution: University of Yamanashi
• Principal Investigator: ITO Sadahiro 山梨大学, 大学院総合研究部, 助教 (30345722)
• Co-Investigator(Kenkyū-buntansha): 長友 啓明 山梨大学, 大学院総合研究部, 講師 (30746813) ; 神沼 修 広島大学, 原爆放射線医科学研究所, 教授 (80342921)
• Project Period (FY): 2018-04-01 – 2024-03-31
• Keywords: アレルギー / 免疫反応の系統差 / 皮膚疾患 / 肥満と突然死

Outline of Final Research Achievements

We initially investigated the 129/Sv//Ev region { The plan was to randomly destroy 20 genes related to immunity from D1Mit36 (76.73cM) to D1Mit115 (82.78cM) using genome editing. Therefore, we performed whole genome sequencing on one homozygous individual.
However, as we continued backcrossing to C57BL/6N and maintained the line, the antinuclear antibody titer no longer increased. Therefore, it is no longer possible to edit the genome using an increase in antinuclear antibody titer as an indicator.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

ホモ個体において、免疫に関係する表現系が現れた。前脚と後脚の間の腹部を体液が出る程掻きむしる個体と肥満して突然死する個体が出てきた。
抗核抗体価の上昇に関わる領域は、129/Sv//Ev領域｛D1Mit36（76.73cM）からD1Mit115（82.78cM）｝の中ではなく、ごく近い近傍であることが強く示唆された。