Study of gene function and its expression for the dipause of annual killiifish

2022 Fiscal Year Final Research Report

• Project/Area Number: 18K06327
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
• Research Institution: The University of Tokyo
• Principal Investigator: KUROKAWA Daisuke 東京大学, 大学院理学系研究科(理学部), 助教 (40342779)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Keywords: 発生休止

Outline of Final Research Achievements

The diapause of vertebrate was analyzed by using annual killifish (Nothobranchius korthausae).
In order to investigate the fuctions of gene whose expression levels increase during diapause, we created mutants of these candidate gene by genome editing using CRISPR/Cas9.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

これまでほとんど研究がされてこなかった脊椎動物の発生休止現象の理解をすすめるための基盤を作った。またこれらの実験を進める中で、比較的飼育の容易な東アフリカ産の一年魚Nothobranchius korthausaeの飼育技術を確立して系統を維持し、トランスジェニック魚の作成やゲノム編集法を確立した。