Studying the effects of activation of intracellular signaling on water and urea permeability in the inner medullary collecting duct

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K08248
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Tohoku Medical and Pharmaceutical University
• Principal Investigator: MORIMOTO TETSUJI 東北医科薬科大学, 医学部, 教授 (10344657)
• Co-Investigator(Kenkyū-buntansha): 根東 義明 日本大学, 医学部, 教授 (00221250) ; 安藤 史顕 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座助教 (80804559)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: 腎髄質内層集合尿細管 / 水透過性 / 尿素透過性

Outline of Final Research Achievements

I planned the following experiments. I dissect inner medullary collecting duct from the mouse kidney and then I will confirm whether protein kinase A anchor protein and protein kinase A affect to the water and urea permeability of this tubule using in vitro microperfusion study. However, it was unexpectedly difficult. I decided to do an alternative experiment using immortal cells. But some troubles happened when I thawed the frozen cells. Finally, I could not produce any research results.

Free Research Field

水電解質

Academic Significance and Societal Importance of the Research Achievements

今回は、研究計画作成時に想定した研究成果が得られなかったため、学術的意義や社会的意義はない。