The elucidation and pathophyological function of endothelial micro particles in sepsis

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K08907
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Tohoku University
• Principal Investigator: Saito Koji 東北大学, 大学病院, 准教授 (10359515)
• Co-Investigator(Kenkyū-buntansha): 山田 充啓 東北大学, 大学病院, 講師 (00396483) ; 武井 祐介 東北大学, 大学病院, 助教 (80822890)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: マイクロパーティクル / 敗血症 / 内皮傷害 / 血管透過性

Outline of Final Research Achievements

Inflammatory stimulation to human umbilical vein endothelial cells (HUVECs) increased EMPs in a concentration-dependent manner. The circulating EMPs in patients with sepsis were significantly increased compared to non-septic patients, reflecting vascular endothelial injury. In addition, PECAM + EMPs and VE-cadherin + EMPs were significantly increased in septic shock patients compared to septic patients. When vascular permeability is changed to HUVECs by permeability modifying agents, PECAM + EMPs and VE-cadherin + EMPs were consistent with changes in vascular permeability and their release was altered, so that PECAM + EMPs and VE-cadherin + EMPs might be novel indicators of vascular permeability in sepsis.

Free Research Field

集中治療

Academic Significance and Societal Importance of the Research Achievements

敗血症において血管内皮傷害は、病態形成に積極的に関与し、かつ血管内皮傷害は臓器傷害の主因となる。本課題により、内皮由来微小粒子（EMPs）は敗血症の血管内皮傷害マーカーとなり得ることをが示された。特にPECAM+EMPsやVE-cadherin+EMPsは、敗血症での血管透過性亢進の指標にもなり得るもので、今後の敗血症の新たな病態マーカーとなり得るものである。