Effect of trancription toward differentiation or proliferation of odontogenic epithelium in oncogenesis within the intraosseous microenvironment

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K09549
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57020:Oral pathobiological science-related
• Research Institution: Tohoku University
• Principal Investigator: KUMAMOTO HIROYUKI 東北大学, 歯学研究科, 教授 (70215028)
• Co-Investigator(Kenkyū-buntansha): 清水 良央 東北大学, 歯学研究科, 助教 (30302152) ; 及川 麻理子 東北大学, 歯学研究科, 助教 (00712902)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 歯原性腫瘍 / 腫瘍発生 / 骨内進展

Outline of Final Research Achievements

Regulator molecules associated with neovascularized condition, development, and cell stemness were examined to clarify the effect of transcription system in
oncogenesis of odontogenic epithelium under the intraosseous microenvironment.
Neovascularization factors, EphA2, EphB4, and EphrinB2, were greater in ameloblastoma than in tooth germ, and measurement of CD34-positive microvessels and D2-40-positive lymphatic vessels was correlated positively with the Eph/Ephrin molecules. Development-related Hippo pathway signaling molecules, YAP and TAZ, were expressed higher in ameloblastoma than in tooth germ, whereas stem cell-associated transcription factors, Sox2 and Klf4, were lower in ameloblastoma than in tooth germ. These transcription factors also showed differences among histological variation and clinical behavior.

Free Research Field

医歯薬学

Academic Significance and Societal Importance of the Research Achievements

正常および腫瘍性の歯原性組織における脈管新生動態、発生の制御分子、幹細胞関連分子を検索し、腫瘍化による歯原性上皮細胞およびその微小環境の特異的な状況や特徴的な腫瘍組織所見・臨床病態との関連を見出した。これらによって、歯原性腫瘍を包括する骨内微小環境の形成要因および同微小環境下での歯原性上皮の増殖や分化に関わる転写調節について、検討することができた。これらの因子は、歯原性上皮が関わる様々な病変の診断や予後判定、治療に応用しうると考えられる。