2021 Fiscal Year Final Research Report

Application of directly converted osteoblasts to disease model animals

Research Project

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Project/Area Number	18K09640
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57040:Regenerative dentistry and dental engineering-related
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Takizawa Shigeta 京都府立医科大学, 医学(系)研究科(研究院), 助教 (70793082)
Co-Investigator(Kenkyū-buntansha)	松田修京都府立医科大学, 医学(系)研究科(研究院), 教授 (00271164) 足立哲也京都府立医科大学, 医学(系)研究科(研究院), 講師 (10613573) 山本俊郎京都府立医科大学, 医学(系)研究科(研究院), 講師 (40347472) 佐藤良樹京都府立医科大学, 医学部附属病院, 専攻医 (50808235) 金村成智京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70204542)
Project Period (FY)	2018-04-01 – 2022-03-31
Keywords	ダイレクト・リプログラミング / 骨芽細胞 / スキャフォールド / 再生療法
Outline of Final Research Achievements	In recent years, techniques for direct reprogramming from fibroblasts to target cells have been reported, and we have succeeded in direct reprogramming from fibroblasts to osteoblasts.However, simply injecting induced osteoblasts is often not sufficient for large bone defects. Therefore, we aimed to develop a novel and effective bone regeneration therapy by developing an appropriate scaffold, performing 3D direct reprogramming of osteoblasts on the scaffold, and transplanting them. The results of this study showed that osteoblasts could be induced directly from fibroblasts on a sheet-type cross-linked nanogel, and that significant bone regeneration was observed when transplanted into a bone defect model mice.
Free Research Field	歯科口腔科学
Academic Significance and Societal Importance of the Research Achievements	難治性で治療方法が確立されていない大規模骨欠損および炎症性骨欠損障害が、患者の線維芽細胞を用いて体外で骨芽細胞移植によって再生し、治療効果が認められれば、有用である。