2022 Fiscal Year Final Research Report

Elucidation of radiation-induced damage to tooth root formation using mouse tooth germ local irradiation model

Research Project

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Project/Area Number	18K09733
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57060:Surgical dentistry-related
Research Institution	The Nippon Dental University
Principal Investigator	Ide Yoshiaki 日本歯科大学, 生命歯学部, 准教授 (70409225)
Co-Investigator(Kenkyū-buntansha)	中原貴日本歯科大学, 生命歯学部, 教授 (10366768) 深田哲也日本歯科大学, 生命歯学部, 助教 (20386254) 那須優則日本歯科大学, 生命歯学部, 教授 (50130688) 中島慎太郎日本歯科大学, 生命歯学部, 助教 (40817095) 春原正隆日本歯科大学, 生命歯学部, 教授 (70287770) 鈴木金吾日本歯科大学, 生命歯学部, 講師 (40318754)
Project Period (FY)	2018-04-01 – 2023-03-31
Keywords	歯根形成障害 / 放射線被曝
Outline of Final Research Achievements	Although it has been reported that radiotherapy in childhood causes secondary shortening of the tooth root, the mechanism has not yet been elucidated. In order to elucidate the mechanism that causes root formation disorder, we observed the morphological changes of mouse tooth roots, the apical region, and histological changes centered on Hertwig's epithelial root sheath (HERS) using an experimental apparatus for local irradiation of mouse tooth germs. In the study, abnormal dentin formation was observed in the apical region, abnormal dynamics of HERS located in the apical region, and disappearance of cytokeratin-positive cells were observed. The disappearance of cytokeratin-positive cells is thought to be HERS mesenchymal transformation (EMT), etc., and further analysis will be performed to clarify the cause of the disappearance.
Free Research Field	解剖学
Academic Significance and Societal Importance of the Research Achievements	小児期において放射線治療が行われると副次的に歯根の短縮が生じると報告されている。放射線が歯の歯根発生に影響を与えることが考えられるが、そのメカニズムの解明までには至っていない。本研究は独自に開発したマウス歯胚局所照射装置を用い、放射線を歯胚に直接照射し、歯根形成への影響を解析した。本研究は、放射線照射により引き起こされる歯根形成阻害の予防法開発の一助となると思われる。