Creation of the plant cultured cells to produce the functional products

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K14389
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: Toyama Prefectural University
• Principal Investigator: Kitaoka Naoki 富山県立大学, 工学部, 助教 (20785547)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: 植物培養細胞 / 遺伝子組換え / タケ

Outline of Final Research Achievements

We generated the transformed bamboo cells which efficiently produced the functional products. Firstly, we generated the bamboo cells which were transformed by hydroxycinnamic acid hydratase/aldolase (PpHCHL) from Pseudomonas putida. In the PpHCHL-transformed cells, the high accumulation of glucose conjugates of vanillic acid and 4-hydroxybenozic acid were observed. Secondary, we generated the bamboo cells which were transformed to express phenolic acid decarboxylase (BaPAD) from Bacillus amyloliquefacience. In the BaPAD transformed cells, primeverosides of 4-vinylpheol and 4-vinylguaiacol were produced.

Free Research Field

生物有機化学

Academic Significance and Societal Importance of the Research Achievements

タケ培養細胞を宿主としてヒドロキシ安息香酸およびビニルフェノールの配糖体を生産可能であることを示した。特に、ヒドロキシ安息香酸のグルコース配糖体の生産量は培地1 Lあたり1.7 gに達し、タケ培養細胞が極めて優れた物質生産宿主であることを示すことができた。
また、ヒドロキシ安息香酸とビニルフェノールで異なる糖が結合した配糖体が得られたことから、タケ培養細胞内における配糖体の生成機構に興味が持たれる。