2020 Fiscal Year Final Research Report
Development of novel breeding techniques using cytoplasmically localized isoforms with unknown functions
| Project/Area Number |
18K14444
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 39010:Science in plant genetics and breeding-related
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| Research Institution | Setsunan University (2020)
Kyushu University (2018-2019) |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 転写開始点選択 / 遺伝子発現制御 / 環境シグナル伝達 / フィトクロム / 光環境応答 / 光受容体 / タンパク質局在 |
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| Outline of Final Research Achievements |
To verify that the light-dependent regulation of transcription start sites discovered in Arabidopsis also exists in rice and tomato, which are practical plants, we performed transcription start site analysis (TSS-seq analysis) using next-generation sequencers for rice and tomato, and found that the light-dependent regulation of transcription start sites also exists in rice and tomato. We found that light-dependent regulation of transcription start sites also exists in rice and tomato.
In addition, we have constructed and prepared binary vectors for the transformation of genes that produce cytoplasmic isoforms by light-dependent transcription start site regulation in rice.
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| Free Research Field |
遺伝育種科学
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| Academic Significance and Societal Importance of the Research Achievements |
イネやトマトでも光依存的な転写開始点制御が行われていたことから、環境に応答したゲノムワイドな転写開始点の制御が様々な植物で行われていることが示唆された。このことから、様々な植物種で環境変化に応答した転写開始点制御によって機能未知の細胞質局在型アイソフォームが生じると考えられる。これらの機能未知の細胞質局在型アイソフォームは植物の環境への適応に働いていると考えられ、環境ストレスへの耐性の付与を目的とした育種への利用が期待される。
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