2020 Fiscal Year Final Research Report
The analysis of the transcriptional regulation for plant growth by CAPTURE approach
| Project/Area Number |
18K14734
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | Nagoya University (2019-2020)
Yokohama City University (2018) |
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Principal Investigator |
Hideki Yoshida 名古屋大学, 生物機能開発利用研究センター, 特任助教 (10814353)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 成長制御 / 転写調節 / CRISPR/Cas9 / 技術開発 |
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| Outline of Final Research Achievements |
As a result of investigating the plasmids, we were able to stably express dCas9 protein and biotinyltransferase in protoplasts by using a specific promoter and an optimized dCas9 gene sequence. We were also able to detect biotinylated dCas9 by incubating protoplasts expressing dCas9 and biotinyltransferase in the specific buffer containing biotin, but we could not confirm precipitation of biotinylated dCas9 by streptavidin.
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| Free Research Field |
植物生理学
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| Academic Significance and Societal Importance of the Research Achievements |
植物において特定のプロモーター領域上に結合している転写制御因子の網羅的な解析は未だに確立されておらず、本研究は最近ヒト培養細胞において確立されたCAPTURE法を利用し、植物において解析を行う試みであった。実際にタンパク質を解析する段階までは進めなかったものの、プロトプラスト内で特定のタンパク質にビオチン化を引き起こすことができる技術は、通常のタグ付きタンパク質発現では得られなかった成果を得られる可能性があり、重要な知見が得られた。
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