2019 Fiscal Year Final Research Report
Development of a novel rapid diagnosis system of fungal infections using functionally-modified recombinant endoglucanase
| Project/Area Number |
18K15151
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Keywords | β-1,6-グルカナーゼ / β-1,6-グルカン / β-D-グルカン / 深在性真菌症 |
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| Outline of Final Research Achievements |
A functionally modified mutant-endoglucanase was generated in Escherichia coli by substituting amino acids that regulate the glycolytic hydrolytic activity of β-1,6-glucanase. Using this, an ELISA-like assay specific for soluble β-1,6-glucans was constructed. The development of this ELISA-like assay has enabled the sensitive determination of trace amounts of β-1,6-glucans produced by various types of Candida species.
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| Free Research Field |
薬学
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| Academic Significance and Societal Importance of the Research Achievements |
真菌に特徴的な多糖構造であるβ-1,6-グルカンを安価かつ高感度に検出する方法を新たに開発したことで，β-1,3-グルカンを指標とする従来法で問題視されていた偽陽性反応を抑えた新しい真菌感染症診断法の開発が期待される。
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