2021 Fiscal Year Final Research Report
Genetic and functional analysis of dental pulp stem cells derived from patients diagnosed with hypoganglionosis aiming for autologous cell transplantation
| Project/Area Number |
18K16260
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Keywords | ヒルシュスプルング病 / ヒルシュスプルング病類縁疾患 / 腸管神経節細胞僅少症 / 腸管不全 / 脱落乳歯歯髄幹細胞 / 再生医療 / 腸管再生 |
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| Outline of Final Research Achievements |
<Characteristics of stem cells derived from hypoganglionois> The proliferation ability and differentiation ability were the same as that of stem cells derived from healthy children.
<Examination of stem cell transplantation> As an examination of the transplantation effect of patient-derived dental pulp stem cells on disease model mice, the observation of the body weight gain after transplantation was finished. Results such as weight gain, changes in intestinal movement, and microscopic changes were obtained. Weight gain was also observed in the patient-derived stem cell transplant group, and intestinal peristalsis showed an improving tendency electrophysiologically. Further analysis will be conducted in the future.
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| Free Research Field |
小児外科
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、ヒルシュスプルング病類縁疾患の患児由来の乳歯幹細胞を用いて自身の腸管蠕動を改善させることを目的とした研究である。既に健常児由来の乳歯幹細胞を用いた疾患モデルマウスの治療効果は証明済みであるため、今後も本研究を継続し、自家細胞移植での治療達成を目指したい。
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