2021 Fiscal Year Final Research Report
Hydrogen inhalation inhibits skeletal muscle damage and ectopic adipocyte accumulation after hindlimb ischemia/reperfusion injury in mice
| Project/Area Number |
18K16401
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 55030:Cardiovascular surgery-related
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Keywords | 下肢虚血再灌流 / 水素ガス |
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| Outline of Final Research Achievements |
A new mouse model of lower limb ischemia-reperfusion was created in which the left hind lower limb of mice was ischemia-conditioned with a bandage. The effect of hydrogen gas on the model was verified by comparing a group that received hydrogen gas with a control group that received air.
Hydrogen gas administration significantly suppressed myocyte necrosis. In the control group, after myocyte necrosis was caused by ischemia, regenerated myocytes and some of them were replaced by adipocytes, but in the hydrogen gas group, most of the normal myocytes were maintained and the appearance of adipocytes was also suppressed. In addition, the infiltration of neutrophils, lymphocytes, and macrophages into inflammatory cells was significantly inhibited, as was IL-6, a proinflammatory cytokine.
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| Free Research Field |
血管外科
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| Academic Significance and Societal Importance of the Research Achievements |
急性下肢動脈閉塞は致死率の高い疾患であるが、致死率の高い原因の一つに、血行再建後の虚血再還流障害があげられる。今回の研究により、下肢虚血再灌流に対して水素ガスを吸引することで炎症が抑制される可能性を示した。実臨床においても、急性下肢動脈閉塞の患者様に水素ガスを吸引させることで、血行再建後の虚血再還流障害を抑制できる可能性が示唆された。
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