Study of the mechanism of cancer pain in nerve tissue-specific fluorescent protein-expressing mice

2022 Fiscal Year Final Research Report

• Project/Area Number: 18K16500
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55050:Anesthesiology-related
• Research Institution: Osaka Medical and Pharmaceutical University
• Principal Investigator: NAKAO KENTA 大阪医科薬科大学, 医学部, 助教 (50815719)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Keywords: がん性疼痛 / 神経栄養因子 / thy1-YFPマウス / 蛍光タンパク / 坐骨神経切断マウス / 骨転移がんモデル

Outline of Final Research Achievements

The purpose of this study is to generate a bone metastasis model of cancer in thy1-YFP mice that specifically express fluorescent proteins in neural tissues, and to clarify the involvement of neurotrophic factors in the germination of nerve fibers distributed in cancer tissues and the formation of neuromas, the expression of genes such as MMP-9 in cancer tissue, dorsal root ganglion and spinal cord, and the pathogenesis of cancer pain. Focusing on the point that neurotrophic factors released in cancer tissues are retrograde axonal transport of primary afferent fibers, the sciatic nerve is cut and the transport is interrupted, and the site of action of the neurotrophic factor is cancer tissue or dorsal root ganglion.

Free Research Field

がん性疼痛

Academic Significance and Societal Importance of the Research Achievements

本研究は、神経栄養因子（NGF）をはじめとする神経栄養因子の遺伝子の発現や形態学的変化に及ぼす影響に焦点を当て、がん性疼痛の発症メカニズムの解明を目的とし、本研究成果はがん患者のQOLの向上につながることが期待できる。