MicroRNA derived from myeloid-derived suppressor cell might contribute to the pathogenesis concerning immunosuppression in sepsis

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K16525
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Kansai Medical University
• Principal Investigator: NISHIMOTO Kota 関西医科大学, 医学部, 助教 (30758850)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: MicroRNA / 骨髄由来抑制細胞 / sepsis / immunosuppression

Outline of Final Research Achievements

In the first year, we evaluated the time course changes of comprehensive microRNA expression by the next-generation sequencing after extraction of blood and peritoneal macrophage of rat sepsis model using lipopolysaccharide injection. Subsequent next year, we have extracted rat myeloid-derived suppressor cell from blood and peritoneal cavity by positive selection of the cell surface marker. Thereafter, we evaluated the time course changes of comprehensive microRNA expression by the next-generation sequencing. Changes in microRNA expression were observed in rat macrophages and myeloid-derived suppressor cells in rats under sepsis compared with rats by saline infusion(control group)
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Free Research Field

集中治療医学

Academic Significance and Societal Importance of the Research Achievements

１つのmiRNAが複数の遺伝子の発現に関与していることから、今まで１つのタンパク質の発現変化で説明が出来なかったCommon Diseaseの病態解明、バイオマーカー、治療薬としてmiRNAの解析は期待できる。報告者らは、敗血症病態時の骨髄由来抑制細胞が産生するmiRNAが重要な役割を担うと考える。