ATP-mediated inflammatory pain in the peripheral trigeminal neurons

2023 Fiscal Year Final Research Report

• Project/Area Number: 18K17179
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Kanagawa Dental College
• Principal Investigator: Kuroda Hidetaka 神奈川歯科大学, 歯学部, 講師 (90755018)
• Project Period (FY): 2018-04-01 – 2024-03-31
• Keywords: 三叉神経節細胞 / 侵害受容性疼痛 / P2X4受容体 / P2X7受容体 / Panx-1チャネル / カルシトニン受容体様受容体 / アデニル酸シクラーゼ / cAMP

Outline of Final Research Achievements

This study investigated the details of intracellular and intercellular communication via ATP receptors in the trigeminal nervous system. It was suggested that rat trigeminal ganglion cells possess the function of ATP autocrine through P2X7 receptors, Panx-1 channels, and P2X4 receptors. Additionally, interactions between trigeminal ganglion cells were observed. In rat brain microvascular endothelial cells, the presence of a signaling pathway mediated by Gs protein-coupled receptors, activated by adenylate cyclase, was suggested.
The findings indicate that intracellular and intercellular communication in trigeminal ganglion cells, mediated by intracellular signaling molecules such as ATP, Ca2+, and cAMP, plays a crucial role in the mechanisms of inflammatory pain.

Free Research Field

外科系歯学（歯科麻酔学）

Academic Significance and Societal Importance of the Research Achievements

本研究は三叉神経の炎症による痛みに、ATPを介した細胞間コミュニケーションが関与する可能性と、周囲の血管におけるシグナル伝達経路がそれらの調節に関与する可能性を明らかにした。
本研究は、炎症性疼痛に対する新たな疼痛治療法の開発や疼痛管理の改善、患者のQOL向上に貢献できた。