Novel treatment and clarifying pathophysiology of BRONJ using cell sheet engineering and next generation sequence

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K17181
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Kaibuchi Nobuyuki 東京女子医科大学, 医学部, 講師 (50621330)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: ビスホスホネート / 顎骨壊死 / デノスマブ / 間葉系幹細胞 / 細胞シート / 再生医療等製品 / 骨粗鬆症 / 骨転移

Outline of Final Research Achievements

We investigated the use of mesenchymal stem cell sheets as a novel treatment for bisphosphonate-related osteonecrosis of the jaw (BRONJ), for which there is still no clear treatment. We had previously confirmed the efficacy in experiments using rats. As a translational research aimed at the clinical study, we conducted an experiment using beagle dogs and showed the same efficacy as the rat experiment. In addition, since the pathogenesis of BRONJ is not fully understood, gingival-derived mesenchymal stem cells collected from patients were comprehensively analyzed for genes by next-generation sequencing. We will report the results in the future.

Free Research Field

口腔外科学、再生医学

Academic Significance and Societal Importance of the Research Achievements

BRONJは骨粗鬆症やがん患者にとって重大な懸念となっているが、明確な治療法が確立しているとは言えず、対応に苦慮している。治療戦略として、軽症例のうちに治癒を得ることが重要であるが、現在の治療のコンセンサスでは根本的な治癒が得られる治療法は確立しているとは言えない。今回の研究で、間葉系幹細胞シートによるBRONJの治療は画期的な新規治療法となり得ることを示した。