Novel cryopreservation method for the effective collection of dental pulp stem cells and culturing dental pulp tissue over transport

2022 Fiscal Year Final Research Report

• Project/Area Number: 18K17183
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Tsurumi University
• Principal Investigator: Takebe Yusuke 鶴見大学, 歯学部, 助教 (50807097)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Keywords: 歯髄幹細胞 / 歯髄組織 / 凍結保存 / 間葉系幹細胞 / 再生医療

Outline of Final Research Achievements

In our study, dental pulp tissue fragments were placed between porous membranes and cultured, and cell migration at the tissue margins was observed. Next, we focused on SDF-1 (stromal derived factor-1) as a mechanism of cell migration, and immunostaining of the tissue fragments showed that SDF-1 was most strongly expressed in the tissue fragments cultured for 72 hours. Furthermore, when the tissue fragments were cultured with a neutralizing antibody against SDF-1, it was confirmed that the migration of cells in the tissue fragments was delayed. In addition, when proteins were extracted from the pulp tissue fragments and analyzed using multiplex assay, it was confirmed that the expression levels of two other factors, GRO-α and MCP-1, increased over time.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

本研究の結果より、多孔性メンブレンで抜去歯より採取した歯髄組織片を挟むことで、輸送中も培養が可能となり、一般開業医などで採取後に、CPC(細胞培養加工施設)などへ輸送することによりオーダーメイドの再生医療の裾野の拡大が期待できる。
また、細胞遊走のメカニズムに関してもSDF-１(stromal derived factor-1)、GRO-α、MCP-１の関連が示唆された。