Elucidation of the functions of IGF1 and Hif1a in tooth development to regulate bioengineered tooth morphogenesis

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K17241
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Tohoku University
• Principal Investigator: Oyanagi Toshihito 東北大学, 大学病院, 助教 (90805326)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: IGF1 / Hif1α / Hif2α / 歯胚発生

Outline of Final Research Achievements

In this study, I examined the expression, function, and interaction between IGF1 and Hif1a in tooth morphogenesis, to acquire fundamental knowledge for regulating bioengineered teeth morphology based on IGF1. As a result, it was revealed that IGF1 regulates the morphology of bioengineered teeth by enhancing the proliferation and differentiation of dental epithelial and mesenchymal cells, as well as inducing enamel knot formation. Furthermore, it was suggested that IGFBP, which is closely related to hypoxia signaling, is involved in the differentiation of dental mesenchymal cells into odontoblasts.

Free Research Field

医歯薬学

Academic Significance and Societal Importance of the Research Achievements

失われた組織や臓器を取り戻すことができる再生医療は、今もなお社会から切望されている。本研究の成果は、IGF1を基軸とした実用的な歯の再生に向けた基盤技術のさらなる発展に寄与するだけでなく、発生過程でIGF1による形態制御を受ける様々な臓器の再生にも応用できる可能性があり、将来の再生医療技術の進歩に役立つ点で非常に大きな意義がある。