2023 Fiscal Year Final Research Report
Modification of energy monitoring signals by advanced glycation end products in diabetes-associated periodontitis
| Project/Area Number |
18K17300
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57080:Social dentistry-related
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Project Period (FY) |
2020-03-01 – 2024-03-31
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| Keywords | 糖尿病関連歯周炎 / 終末糖化産物 / RAGE / AMP活性プロテインキナーゼ |
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| Outline of Final Research Achievements |
To investigate the association between periodontal disease and AGEs accumulation, the effects of short-term AGE-bovine serum albumin (BSA) on cell proliferation and cell death were evaluated in the human gingival epithelium-derived cell line Ca9-22 and the mouse osteoblast-like cell line MC3T3-E1. Each cell line showed a tendency to inhibit cell proliferation, but no significant difference was observed with respect to increased cell death compared to control BSA. In addition to these cell lines, the expression of RAGE, the receptor for AGEs, and whether it is induced by AGEs in mouse salivary glands and other organs was examined at mRNA and protein levels. The effects of AGEs on periodontal tissues did not appear to be via a receptor-mediated mechanism.
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| Free Research Field |
予防歯科学
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| Academic Significance and Societal Importance of the Research Achievements |
歯周病は糖尿病の合併症の１つであるが、この２つの疾病の間の相互関係や因果関係についてはいまだに解明されていない。近年、この病態に慢性的な高血糖によるタンパク質の糖化反応で生じる終末糖化産物（AGEs）の蓄積が関与していることが示唆されている。
歯周組織のAGEs蓄積の非侵襲的な評価法を確立するため、研究者および研究協力者の唾液を用いて試験的研究を行った。ELISA法により、唾液中にAGEsが含まれていることがわかった。また、唾液中のどのタンパク質が糖化されているかを検討したが、明確な結果を得られなかった。
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