Induction of cancer stem cells in pancreatic cancer cells and their treatment with radiosensitized nanoparticles

2022 Fiscal Year Final Research Report

• Project/Area Number: 18K18368
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Kobe University
• Principal Investigator: Nishimura Yuya 神戸大学, 科学技術イノベーション研究科, 客員准教授 (40728218)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Keywords: 幹細胞 / 人工がん幹細胞 / すい臓がん / 放射線増感 / ナノ粒子

Outline of Final Research Achievements

We established induced cancer stem cells (iCSCs) using pancreatic cancer cells. As a result, morphological changes similar to iPS cells were observed in one cell line. In the cell group, expression of cancer stem cell marker genes was confirmed. Transcriptome analysis by microarray and metabolome analysis by LCMSMS of this cell group confirmed that several genes and metabolites were significantly increased. As a result of evaluating whether antibodies targeting these marker candidates specifically recognize iCSCs, increased antibody binding was confirmed compared to control cells. Therefore, a target marker for pancreatic cancer induced cancer stem cells was suggested.

Free Research Field

生物工学

Academic Significance and Societal Importance of the Research Achievements

これまで取得が困難であったがん幹細胞を人工的に作製することにより、学術的な研究は一気に進むことが考えられる。がん幹細胞の細胞数を確保できることで、これまで不可能であった代謝解析も十分に実施可能となる。また、すい臓がんの人工がん幹細胞が初めて作製されたことで、すい臓がんの治療を目的とする研究に与える影響は大きい。さらに、このすい臓がん人工がん幹細胞のマウスへの移植が確立すれば、これまでのがん細胞株移植マウスとは次元の違う評価が可能と思われる。