Noncontact wettability sensing and cell quality control

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K18374
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tanaka Nobuyuki 国立研究開発法人理化学研究所, 生命機能科学研究センター, 上級研究員 (00724692)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: 濡れ性 / 非接触センシング / 細胞品質 / 界面分子挙動

Outline of Final Research Achievements

In this study, the principle of non-contact wettability sensing, which is the applicant's original technology, was deepened and applied to cell quality evaluation. A jet of air compressed to a certain pressure was applied to a surface covered with liquid to temporarily remove the liquid. Cultured cells of the mouse skeletal myoblast cell line C2C12 were cultured in serum-added medium for cell proliferation and serum-free medium for myotube differentiation induction, respectively. The results showed that the liquid recovery time after cessation of air jetting was prolonged after each day of culture when differentiation was induced, compared to serum-added medium. It can be concluded that the successive changes in cell differentiation can be detected by non-contact wettability sensing.

Free Research Field

機械工学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、これまで非接触濡れ性センシングが、単なる巨視的な濡れ現象の評価手法というだけでなく、濡れ現象を介した非破壊的な細胞分化検出系に応用可能であり、幹細胞生物学や再生医療における新たな細胞評価系への展開が期待できる学術的・社会的意義を示すものである。