Chemical regulation of the transport substrate switching phenomenon in the plant multifunctional transporter GTR1

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K19137
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 37:Biomolecular chemistry and related fields
• Research Institution: Tohoku University
• Principal Investigator: Ueda Minoru 東北大学, 理学研究科, 教授 (60265931)
• Project Period (FY): 2018-06-29 – 2021-03-31
• Keywords: 植物ホルモン / 輸送体 / ジャスモン酸 / ジベレリン

Outline of Final Research Achievements

The plant membrane transporter protein GTR1 was first discovered as a glucosinolate transporter, but was later found to also function as a transporter of two plant hormones. In order to elucidate the mechanism of using these structurally completely different substrates, we evaluated various transport activities using mutant proteins and an evaluation system using African clawed frog oocytes. The results suggest that GTR1 has the ability to transport both as a dimer and as a monomer, but that there are multimers corresponding to the substrates.

Free Research Field

天然物ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

植物の生理応答をコントロールする植物ホルモンの分布の時空間制御は、植物の機能制御を考える上で極めて重要である。その意味で、植物ホルモン輸送体の基質輸送と基質選択性をコントロールできれば、大きな社会的意義がある。一般的に輸送体の活性を制御する化学物質の開発は難しいが、今回の結果はそれに繋がる可能性をもち、学術的にも興味深い。