2020 Fiscal Year Final Research Report
Preliminary study on diagnosis of fatal arrhythmia in forensic autopsy
| Project/Area Number |
18K19694
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 58:Society medicine, nursing, and related fields
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Keywords | 突然死 / 致死性不整脈 / 急性心筋梗塞 / HO-1 |
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| Outline of Final Research Achievements |
We examined the immunohistochemical expression of Heme oxygenase-1 (HO-1) in the heart samples to discuss their forensic significance to determine acute cardiac ischemia. The heart samples were obtained from 23 acute ischemic heart diseases (AIHD) cases and 33 non-AIHD cases as controls. HO-1 positive signals in cardiomyocyte nuclear were detected in 78.2% of AIHD cases, however, that were detected in only 24.2% control cases with statistical difference between AIHD and non-AIHD groups. In contrast to HO-1 protein expression, there was no significant difference in the appearance of myoglobin pallor regions and leukocyte infiltration in the hearts between AIHD and non-AIHD groups. From the viewpoints of forensic pathology, intracardiac HO-1 expression would be considered a valuable marker to diagnose AIHD as the cause of death.
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| Free Research Field |
法医学
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| Academic Significance and Societal Importance of the Research Achievements |
法医実務において死因確定は最も重要な実務の一つであり，その際，客観性且つ正確性が求められる．致死性不整脈の法医診断法については未解決で，その最大の理由は生前に致死性不整脈が生じていたか否かの客観的証拠が極めて乏しいものであるからである．突然死の死因の約半数は，心臓・大血管系疾患で占められている．しかしながら，心臓性突然死例と考えられる事例で形態学的に明らかな変化を伴わない，心室細動等の「致死性不整脈」で死亡したと推測される事例にしばしば遭遇する．本研究では，酸化ストレスによる細胞傷害に関与するHO-1の心臓における発現を，心臓死と非心臓死群で検討した．
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