Development of virus-binding protein-based technologies for concentration, detection and identification of pathogenic viruses in water environment

2011 Fiscal Year Final Research Report

• Project/Area Number: 19106009
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: Tohoku University
• Principal Investigator: OMURA Tatsuo 東北大学, 大学院・工学研究科, 教授 (30111248)
• Co-Investigator(Kenkyū-buntansha): KATAYAMA Hiroyuki 東京大学, 大学院・工学系研究科, 准教授 (00302779) ; SANO Daisuke 北海道大学, 大学院・工学研究院, 准教授 (80550368) ; WATANABE Toru 山形大学, 農学部, 准教授 (10302192) ; MASAGO Yoshifumi 東北大学, 大学院・工学研究科, 助教 (50507895)
• Co-Investigator(Renkei-kenkyūsha): UEKI Yo 宮城県保健環境センター, 微生物部, 副主任研究員 (60501062)
• Project Period (FY): 2007 – 2011
• Keywords: 感染症 / ウイルス / タンパク質 / 酵素 / 検出技術

Research Abstract

The objective of this project was to develop novel technologies for recovery, detection and identification of pathogenic viruses in water environments by taking advantages of our own approaches including virus-binding protein (VBP)and enzymatic virus elution (EVE)method. As a result, enteric virus-binding protein (EVBP)that can capture diverse enteric viruses was successfully identified, and novel methodologies for virus recovery from environmental matrixes such as river and estuarine sediments were established. Furthermore, a novel methodology for detecting infectious pathogenic viruses in water by using ethidium monoazide was also developed. These achievements allow us to complete the original targets of this project.

Research Products

• [Journal Article] Adsorption characteristics of an enteric virus-binding protein to norovirus, rotavirus and poliovirus (2011)
  • Author(s): Imai, T., D. Sano, T. Miura, S. Okabe, K. Wada, Y. Masago and T. Omura
  • Journal Title: BMC Biotechnology Volume: 11 Pages: 123
  • Peer Reviewed
• [Journal Article] Development of an effective method of viral genomic RNA recovery from environmental silty sediments for quantitative molecular detection (2011)
  • Author(s): Miura, T., Y. Masago, D. Sano and T. Omura
  • Journal Title: Applied and Environmental Microbiology Volume: 77(12) Pages: 3975-3981
  • Peer Reviewed
• [Journal Article] Quantification and genotyping of human sapovirus in the Llobregat River catchment (2011)
  • Author(s): Sano, D., U. Perez, S. Guix, R. M. Pinto, T. Miura, S. Okabe and A. Bosch
  • Journal Title: Spain. Applied and Environmental Microbiology Volume: 77(3) Pages: 1111-1114
  • Peer Reviewed
• [Journal Article] Detection of oxidative damages on viral capsid protein for evaluating structural integrity and infectivity of human norovirus (2010)
  • Author(s): Sano, D., R. M. Pinto, T. Omura and A. Bosch
  • Journal Title: Environmental Science and Technology Volume: 44(2) Pages: 808-812
  • Peer Reviewed
• [Journal Article] Norovirus-binding proteins recovered from activated sludge microorganisms with an affinity to a noroviral capsid peptide (2010)
  • Author(s): Sano, D., K. Wada, T. Imai, Y. Masago and T. Omura
  • Journal Title: Journal of Applied Microbiology Volume: 109(6) Pages: 1923-1928
  • Peer Reviewed
• [Journal Article] Detection of sapovirus in oysters (2010)
  • Author(s): Ueki, Y., M. Shoji, Y. Okimura, Y. Miyota, Y. Masago, T. Oka, K. Katayama, N. Takeda, M. Noda, T. Miura, D. Sano and T. Omura
  • Journal Title: Microbiology and Immunology Volume: 54 Pages: 483-486
  • Peer Reviewed
• [Journal Article] Development of a real-time RT-PCR assay combined with ethidium monoazide treatment for RNA viruses and its application to detect viral RNA after heat exposure. (2010)
  • Author(s): Kim, K., H. Katayama, M. Kitajima, Y. Tohya and S. Ohgaki
  • Journal Title: Water Science and Technology Volume: 63(3) Pages: 502-507
  • Peer Reviewed
• [Journal Article] Chlorine inactivation of human norovirus, murine norovirus and poliovirus in drinking water (2010)
  • Author(s): Kitajima, M, Y. Tohya, K. Matsubara, E. Haramoto, E. Utagawa and H. Katayama
  • Journal Title: Letters in Applied Microbiology Volume: 51(1) Pages: 119-121
  • Peer Reviewed
• [Journal Article] Enzymatic Virus Elution法を用いた流入下水からのウイルス検出技術の開発 (2009)
  • Author(s): 村田有紗, 真砂佳史, 三浦尚之, 今井崇博, 大村達夫
  • Journal Title: 環境工学研究論文集 Volume: 46 Pages: 197-203
  • Peer Reviewed
• [Journal Article] 培養できない腸管系ウイルス不活化評価を目的とした外殻タンパク質酸化傷害検出手法の開発 (2009)
  • Author(s): 佐野大輔, R. M. Pinto, 大村達夫, A. Bosch
  • Journal Title: 環境工学研究論文集 Volume: 46 Pages: 423-428
  • Peer Reviewed
• [Journal Article] 水環境及び感染性胃腸炎患者から得られたノロウイルスカプシドタンパク質遺伝子の多様性及びアミノ酸配列変異の解析 (2008)
  • Author(s): 今井崇博, 佐野大輔, 真砂佳史, 大村達夫
  • Journal Title: 環境工学研究論文集 Volume: 45 Pages: 355-360
  • Peer Reviewed
• [Journal Article] Enzymatic Virus Elution法によるカキ中腸腺からのウイルス誘出技術の開発 (2008)
  • Author(s): 奥村千恵, 真砂佳史, 佐野大輔, 植木洋, 大村達夫
  • Journal Title: 環境工学研究論文集 Volume: 45 Pages: 179-186
  • Peer Reviewed
• [Journal Article] New tools for the study and direct surveillance of viral pathogens in water (2008)
  • Author(s): Bosch, A., S. Guix, D. Sano and R. M. Pinto
  • Journal Title: Current Opinion in Biotechnology Volume: 19 Pages: 295-301
  • Peer Reviewed
• [Journal Article] Persistence of Caliciviruses in artificially contaminated oysters during depuration (2007)
  • Author(s): Ueki, Y., M. Shoji, A. Suto, T. Tanabe, Y. Okimura, Y. Kikuchi, N. Saito, D. Sano, and T. Omura
  • Journal Title: Applied and Environmental Microbiology Volume: 73(17) Pages: 5698-5701
  • Peer Reviewed
• [Journal Article] 活性汚泥細菌から分離されたノロウイルス吸着タンパク質 (Norovirus-Binding Proteins : NoVBPs)の特性評価 (2007)
  • Author(s): 和田圭史, 佐野大輔, 今井崇博, 大村達夫
  • Journal Title: 水環境学会誌 Volume: 30(12) Pages: 731-736
  • Peer Reviewed
• [Journal Article] Detection of human sapovirus in clams (2007)
  • Author(s): Hansman, G. S., T. Oka, R. Okamoto, T. Nishida, M. Noda, D. Sano, Y. Ueki, T. Imai, T. Omura, O. Nishio, H. Kimura and N. Takeda
  • Journal Title: Japan.Emerging Infectious Diseases Volume: 13 Pages: 620-622
  • Peer Reviewed
• [Journal Article] Sapovirus in water (2007)
  • Author(s): Hansman, G. S., D. Sano, Y. Ueki, T. Imai, T. Oka, K. Katayama, N. Takeda and T. Omura
  • Journal Title: Japan. Emerging Infectious Diseases Volume: 13 Pages: 113-135
  • Peer Reviewed
• [Presentation] Oysters accumulating noroviruses as a tool for disease surveillance system. (2011)
  • Author(s): Ueki, Y., Y. Takahashi, M. Abe, Y. Sato, Y. Okimura, Y. Masago and T. Omura.
  • Organizer: 16th International Symposium on Health-Related Water Microbiology
  • Place of Presentation: Rotorua, New Zealand
  • Year and Date: 20110918-23
• [Presentation] Virus binding-protein with an affinity to multiple genotypes of human norovirus. (2011)
  • Author(s): Imai, T., D. Sano, T. Miura, S. Okabe, Y. Masago and T. Omura.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110906-16
• [Presentation] Human enteric bacteria that capture norovirus particles with a specific interaction through history-blood group antigen-like moiety (2010)
  • Author(s): Sano, D., A. Suenaga, T. Nakagomi, O. Nakagomi and S. Okabe.
  • Organizer: The 2nd COST 929 Symposium
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 20101007-09
• [Presentation] Accumulation of oxidative damages on rotavirus capsid protein and its relationship with infectivity (2010)
  • Author(s): Sano, D., K. Tojo, T. Nakagomi, O. Nakagomi, R. M. Pinto, A. Bosch and S. Okabe.
  • Organizer: The 2nd COST 929 Symposium
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 20101007-09
• [Presentation] Norovirus-binding bacteria : significance in the environmental dissemination of gastroenteritis viruses (2010)
  • Author(s): Sano, D.
  • Organizer: The 44th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program
  • Place of Presentation: Sapporo, Japan.
  • Year and Date: 20100628-30
• [Presentation] Genetic diversity and evolution in amino acid sequences of norovirus genogroup II strains in environmental and clinical samples. (2009)
  • Author(s): Imai, T., D. Sano, Y. Ueki, Y. Masago and T. Omura.
  • Organizer: The 15th Symposium on Health-Related Water Microbiology
  • Place of Presentation: Naxos, Greece.
  • Year and Date: 20090531-0605
• [Presentation] Detection of oxidative damages on capsid proteins of norovirus : a new approach to evaluate the infectivity of non-culturable virus. (2009)
  • Author(s): Sano, D., R. M. Pinto, T. Omura and A. Bosch.
  • Organizer: The 15th Symposium on Health-Related Water Microbiology
  • Place of Presentation: Naxos, Greece.
  • Year and Date: 20090531-0605
• [Presentation] Prevalence and genotypes of Sapovirus in wastewater, oysters and gastroenteritis patients in Japan. (2009)
  • Author(s): Ueki, Y., H. Uemura, Y. Okimura, Y. Masago, T. Miura, T. Omura and K. Miyota.
  • Organizer: 15th International Symposium on Health-Related Water Microbiology
  • Place of Presentation: Naxos, Greece.
  • Year and Date: 20090531-0605
• [Presentation] New detection method for enteric viruses in digestive diverticulum of pacific oyster, Crassostrea gigas utilizing enzymes for virus elution. (2009)
  • Author(s): Masago, Y., C. Okumura, D. Sano, Y. Ueki and T. Omura.
  • Organizer: 15th International Symposium on Health-Related Water Microbiology
  • Place of Presentation: Naxos, Greece.
  • Year and Date: 20090531-0605
• [Presentation] Development of ethidium monoazide (EMA)-RT-PCR for selective detection of enteric viruses (2009)
  • Author(s): Kim, K., H. Katayama, M. Kitajima, Y. Tohya and S. Ohgaki.
  • Organizer: 15th International Symposium on Health-Related Water Microbiology
  • Place of Presentation: Naxos, Greece.
  • Year and Date: 20090531-0605
• [Presentation] Health risk evaluation of Norovirus via oysters for raw consumption. (2009)
  • Author(s): Masago, Y., Y. Ueki and T. Omura
  • Organizer: 6th Netherlands-Japan Workshop on Water Technology
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 2009-10-15
• [Presentation] Possible seasonality in prevalence of viral gastroenteritis in the Metro-Manila (2009)
  • Author(s): Masago, Y., C. Okumura, T. Miura, Y. Ueki, A. Suzuki, M. Saito, L. T. Sombrero, S. P. Lupisan, H. Oshitani, R. M. Olveda and T. Omura.
  • Organizer: the Philippines. International Joint Forum on Infectious Diseases
  • Place of Presentation: Bangkok, Thailand.
  • Year and Date: 2009-09-16
• [Presentation] Enteric infectious diseases in the Metro-Manila, the Philippines estimated from enteric viruses in water and bivalve shellfish. (2008)
  • Author(s): Masago, Y., C. Okumura, Y. Ueki, A. Suzuki, L. Sombrero, S. Lupisan, H. Oshitani, R. Olveda and T. Omura.
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections
  • Place of Presentation: Sapporo, Japan.
  • Year and Date: 20081215-16
• [Presentation] Genetically diverse strains of norovirus genogroup II in water environment. (2007)
  • Author(s): Imai, T., D. Sano, Y. Ueki, K. Fukushi and T. Omura.
  • Organizer: The 2nd International Water Association Asia-Pacific Regional Group Conference & Exhibition.
  • Place of Presentation: Perth, Australia.
  • Year and Date: 20071028-1101
• [Presentation] Affinity isolation of Norovirus-binding proteins from activated sludge microorganisms using a surface-exposed part of viral capsid protein. (2007)
  • Author(s): Sano, D., K. Wada, K. Fukushi and T. Omura.
  • Organizer: 14th International symposium on health-related water microbiology.
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 2007-09-10
• [Book] In George V. Kurladze (ed.), Environmental Microbiology Research Trends (2008)
  • Author(s): Sano, D. and T. Omura.
  • Total Pages: 257-272
  • Publisher: Virus-binding proteins in water environments : natural ligands for human viruses