2009 Fiscal Year Final Research Report
Construction of a homozygous mutant embryonic stem cell bank
| Project/Area Number |
19300146
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
HORIE Kyoji Osaka University, 医学系研究科, 准教授 (30333446)
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| Project Period (FY) |
2007 – 2009
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| Keywords | ES細胞 / マウス / トランスポゾン / レトロウイルス / 遺伝子 / Bloom / 変異体 / ゲノム |
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| Research Abstract |
Mutagenesis of mouse embryonic stem (ES) cells provides a powerful resource for functional analyses of genes. Emerging cell culture techniques for directed differentiation of ES cells will further accelerate this trend. The diploid nature of the mammalian genome often hampers this process because both alleles need to be mutated for each gene. In the present study, we develop a novel method to rapidly mutate diploid genome of mouse ES cells and generate homozygous mutants. This method will facilitate gene function study in tissue culture systems.
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