2009 Fiscal Year Final Research Report
Developments of Fluorescent Probes for Elucidation of Biological Functions of Phosphate Species
| Project/Area Number |
19350085
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto University |
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Principal Investigator |
OJIDA Akio Kyoto University, 大学院・工学研究科, 講師 (10343328)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 蛍光プローブ / バイオセンサー / バイオイメージング / リン酸アニオン / タンパク質 |
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| Research Abstract |
In this research project, we have developed the new fluorescent probes, which are useful for elucidation of biological functions of various biological phosphate species. We especially focused on the developments of the three types of the fluorescent probes, including 1) protein-based hybrid-type biosensor for phosphorylated protein/peptide, 2) molecular probe for aggregation of phosphorylated tau protein exisiting in brain of Alztheimer's disease, and 3) molecular probe for intracellular ATP with a OFF/ON-type fluorescence sensing mode. As the results, we have successfully achieved the probes with the desirable functions in each of the three themes.
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