2010 Fiscal Year Final Research Report
Development and application of cell-free molecular display system
| Project/Area Number |
19360373
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
NAKANO Hideo Nagoya University, 生命農学研究科, 教授 (00237348)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Yugo 名古屋大学, 大学院・生命農学研究科, 准教授 (50273214)
KOJIMA Takaaki 名古屋大学, 大学院・生命農学研究科, 助教 (40509080)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAJIMA Mitsutoshi 筑波大学, 大学院・生命環境科学研究科, 教授 (10343815)
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| Project Period (FY) |
2007 – 2010
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| Keywords | エマルジョンPCR / 無細胞タンパク質合成系 / マイクロチャネル乳化ペプチド / ビーズディスプレイ |
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| Research Abstract |
A novel biomolecule selection system named 'Bead Display' that displays DNA and its encoding protein on the surface of the beads has been developed. Since the system can utilize cell sorter that can analyze and select samples, ultra high-throughput assay at most 10 million samples per an hour can be theoretically possible. As an example, selection of peptides, enzyme, or analysis of DNA binding motifs were successfully demonstrated.
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[Journal Article] Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display.2010
Author(s)
Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H., Nakano, H.
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Journal Title
J.Biosci.Bioeng. 109
Pages: 411-417
Peer Reviewed
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