2010 Fiscal Year Final Research Report
Development of a method to detect intrinsic mRNA in a living cell by using a molecular beacon-immobilized nanoneedle
| Project/Area Number |
19360379
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
NAKAMURA Chikashi National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門・研究グループ, グループ長 (40357661)
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| Project Period (FY) |
2007 – 2010
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| Keywords | ナノニードル / 細胞操作 / mRNA / AFM / モレキュラービーコン |
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| Research Abstract |
We have been developing a low invasive cell manipulation technology using a nanoneedle. In this study, a detection technique of the intracellular mRNA in a living cell using a nanoneedle functionalized with molecular beacons has been developed. It was clarified that intracellular GAPDH mRNAs could hybridize with molecular beacons on the nanoneedle very rapidly and the capability of sequence recognition was very high.
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[Journal Article] Evaluation of the effectiveness of tapered silicon and novel diamond nanoneedles in manipulations of living single cells2009
Author(s)
Sung-Woong Han, Seunghwan Ryu, Taro Kitagawa, Hiroshi Uetsuka, Naoji Fujimori, Yukihiro Aoki, Ryo Ota, Yosuke Amemiya, Nobuo Shimamoto, Chikashi Nakamura, Jun Miyake
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Journal Title
Archives of Histology and Cytology 72(4/5)
Pages: 261-270
Peer Reviewed
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