Translation arrest and degradation of aberrant protein induced by translation of poly(A) tail

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Nagoya University
• Principal Investigator: INADA Toshifumi Nagoya University, 大学院・理学研究科, 准教授 (40242812)
• Project Period (FY): 2007 – 2009
• Keywords: 遺伝子発現 / 品質管理 / 異常mRNA / 異常翻訳 / プロテアソーム / リボソーム / 新生ポリペプチド鎖 / 翻訳アレスト

Research Abstract

Gene expression is highly accurate due to quality-control systems that prevent the expression of potentially harmful protein products. We previously reported that translation of the poly(A) tail plays a crucial role in repressing the production of aberrant proteins from nonstop mRNAs by both translation repression and proteasome-dependent nascent protein destabilization in yeast. This clearly indicates that translation arrest and protein degradation, in addition to mRNA degradation, are crucial for repressing the expression of nonstop mRNAs. We also recently reported that translation arrest caused by the presence of consecutive basic amino acids in a nascent protein induces Not4p-dependent co-translational protein degradation by the proteasome.

Research Products

• [Journal Article] Upf1p stimulates proteasome-mediated degradation of the product derived from the specific nonsense-containing mRNA (2009)
  • Author(s): Kuroha, K., Tatematsu, T., Inada, T
  • Journal Title: EMBO report 10 Pages: 1265-1271
• [Journal Article] Nascent peptide-dependent translation arrest leads to not4p-mediated protein degradation by the proteasome (2009)
  • Author(s): Kuroha, K., Dimitrova, L., Tatematsu, T., Inada, T
  • Journal Title: J. Biol. Chem 284 Pages: 10343-10352
• [Journal Article] Nucleolar structure and function regulated by a deubiquitinating enzyme USP36 (2009)
  • Author(s): Endo, A., Matsumoto, M., Inada, T., Yamamoto, A., Nakayama, K.I., Kitamura, N., Komada, M
  • Journal Title: J. Cell Sci 122 Pages: 678-685
• [Journal Article] Analysis of nonstop mRNA translation in vivo (2009)
  • Author(s): Kuroha, K., Horiguchi, N., Aiba, H., Inada, T
  • Journal Title: Genes to Cells 14 Pages: 739-749
• [Journal Article] Semaphorin controls epidermal morphogenesis by stimulating mRNA translation via eIF2a in Caenorhabditis elegans (2008)
  • Author(s): Nukazuka, A., Fujisawa, H., Inada, T., Oda, Y., Takagi, S
  • Journal Title: Genes Dev 22 Pages: 1025-1036
• [Journal Article] Translation of poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast (2007)
  • Author(s): Ito-Harashima, S., Kuroha, K., Tatematsu, T., Inada, T
  • Journal Title: Genes Dev 21 Pages: 519-524
• [Presentation] RNA制御学 (2009)
  • Author(s): 井上邦夫、稲田利文
  • Organizer: 日本分子生物学会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 20091200
• [Presentation] 新生ポリペプチド鎖依存翻訳アレスト因子RACK1の同定 (2009)
  • Author(s): 稲田利文
  • Organizer: 日本生物化学会シンポジウム「RNA制御学」
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] RNA制御学 (2009)
  • Author(s): 稲田利文、大野睦人
  • Organizer: 日本生物化学会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] The Ribosomes (2009)
  • Author(s): 稲田利文
  • Organizer: MA
  • Place of Presentation: USA
  • Year and Date: 2009-06-06
• [Presentation] 拡大する品質管理の概念 (2008)
  • Author(s): 遠藤斗志也、稲田利文
  • Organizer: 本分子生物学会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 20081200
• [Presentation] Nascent peptide-dependent translation arrest leads to Not4p-mediated protein degradation by the proteasome (2008)
  • Author(s): 稲田利文
  • Organizer: 日本分子生物学会シンポジウム「拡大する品質管理の概念」
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Novel mechanisms for quality control to repress abnormal products from aberrant mRNAs in yeast (2008)
  • Author(s): 稲田利文
  • Organizer: 英最先端シンポジウム(UK-JFOS2008)
  • Place of Presentation: 湘南国際村
  • Year and Date: 2008-10-05
• [Presentation] 40S ribosome-bound RACK1 functions in nascent peptide-dependent translation arrest that leads to Hbs1/Dom34-independent NGD (2008)
  • Author(s): 稲田利文
  • Organizer: Translational Control
  • Place of Presentation: CSHL, USA
  • Year and Date: 2008-09-05
• [Presentation] 遺伝子発現の正解性を保証するmRNA品質管理機構 (2008)
  • Author(s): 稲田利文
  • Organizer: 第18回酵母合同シンポジウム2008
  • Place of Presentation: 甲南大学
  • Year and Date: 2008-05-08
• [Presentation] 生命現象の基盤である正確な遺伝子発現を保証する機構 (2007)
  • Author(s): 稲田利文
  • Organizer: 第23回Wakoワークショップ「mRNA品質管理と疾患」
  • Place of Presentation: 東京
  • Year and Date: 2007-11-16
• [Presentation] Upf1p stimulates nonsense-mediated co-translational protein degradation by the proteasome in yeast (2007)
  • Author(s): 稲田利文
  • Organizer: Translational Control
  • Place of Presentation: EMBL、Germany
  • Year and Date: 2007-09-15
• [Presentation] Consecutive basic residues induce translation arrest and protein degradation by the proteasome in yeast」 (2007)
  • Author(s): 稲田利文
  • Organizer: Translational Control
  • Place of Presentation: EMBL, Germany
  • Year and Date: 2007-09-13
• [Book] 蛋白質核酸酵素増刊号『多様性と非対称性を獲得するRNAプログラム』(概論:遺伝子発現制御の中核をなすRNAプログラム) (2009)
  • Author(s): 稲田利文
• [Book] 多様性と非対称性を獲得するRNAプログラム(RNAプログラムを支える品質管理機構) (2009)
  • Author(s): 稲田利文
• [Book] RNAの機能解明と医療応用(実験医学) (2008)
  • Author(s): 稲田利文
  • Total Pages: 1644-1649
• [Book] mRNAの動態とプロテアソーム(タンパク質の分解機構) (2008)
  • Author(s): 稲田利文
• [Book] RNA実験ノート上巻 (2008)
  • Author(s): 稲田利文、塩見春彦
  • Publisher: 羊土社
• [Book] 多様性と非対称性を獲得するRNAプログラム(蛋白質核酸酵素増刊号)
  • Author(s): 稲田利文、大野睦人
• [Remarks]
  • URL: http://web.me.com/toshiinada/INADA_group_site/Welcome.html