2009 Fiscal Year Final Research Report
Library constructionand biological activities of high mannose N-glycan-specific algal lectins
| Project/Area Number |
19380122
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
HORI Kanji Hiroshima University, 大学院・生物圏科学研究科, 教授 (50116662)
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| Project Period (FY) |
2007 – 2009
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| Keywords | レクチン / 藻類 / 高マンノース型糖鎖 / 生物活性 |
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| Research Abstract |
We succeeded in the cDNA cloning and preparations of active recombinants of high mannose N-glycan-specific algal lectins thatcan be grouped into three types (Type I～III) based on the recognition moiety within the branched oligomannosides of the N-glycans. Furthermore,we newly isolated and characterized the lectins, which belong to type I, from the three cultivated species of edible seaweeds. We found that the type I～III lectins had the anti-HIV activity and inhibitory activity against the growth of the cultured cells which are derived from human colonic cancer. These lectins were also useful to separate easily the high mannose N-glyans to each other from their mixture.
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