Novel roles of lysolipid receptors as mediators of lipoprotein actions and proton sensors

2008 Fiscal Year Final Research Report

• Project/Area Number: 19390015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Gunma University
• Principal Investigator: OKAJIMA Fumikazu Gunma University, 生体調節研究所, 教授 (30142748)
• Project Period (FY): 2007 – 2008
• Keywords: 脂質シグナル分子 / リポ蛋白質 / pH / G蛋白共役受容体

Research Abstract

リゾ脂質性G蛋白質共役受容体をリポ蛋白質作用の仲介機能ならびに細胞外pHセンサー機能という新しい視点から解析した。その結果、血管内皮細胞や平滑筋細胞における様々なリポ蛋白質作用がスフィンゴシン1-リン酸受容体やリゾホスファチジン酸受容体を介していること、また、細胞外pH低下による骨芽細胞のCOX-2発現におけるOGR1受容体の役割などを明らかにした。

Research Products

• [Journal Article] Involvement of Proton-Sensing TDAG8 in Extracellular Acidification-Induced Inhibition of Pro-Inflammatory Cytokine Production In Peritoneal Macrophages (2009)
  • Author(s): Mogi C, Tobo M, Tomura H, Murata N, He XD, Sato K, Kimura T, Ishizuka T, Sasaki T, Sato T, Kihara Y, Ishii S, Harada A, Okajima F
  • Journal Title: J Immunol 182 Pages: 3243-3251
  • Peer Reviewed
• [Journal Article] LPA_1 receptors mediate stimulation, whereas LPA_2 receptors mediate inhibition, of migration of pancreatic cancer cells in response to lysophosphatidic acid and malignant ascites (2009)
  • Author(s): Komachi M, Tomura H, Malchinkhuu E, Tobo M, Mogi C, Yamada T, Kimura T, Kuwabara A, Ohta H, Im DS, Kurose H, Takeyoshi I, Sato K, Okajima F
  • Journal Title: Carcinogenesis 30 Pages: 457-465
  • Peer Reviewed
• [Journal Article] Signaling pathways involved in DNA synthesis and migration in response to lysophosphatidic acid and low-density lipoprotein in coronary artery smooth muscle cells (2009)
  • Author(s): Komachi M, Damirin A, Malchinkhuu E, Mogi C, Tobo M, Ohta H, Sato K, Tomura H, Okajima F.
  • Journal Title: Vasc Pharmacol 50 Pages: 178-184
  • Peer Reviewed
• [Journal Article] Anti-atherogenic actions ohigh-density lipoprotein through sphingosine 1-phosphate receptors and scavenger receptor class B type I (2009)
  • Author(s): Okajima F, Sato K, Kimura T
  • Journal Title: Endocrine J (in press)
  • Peer Reviewed
• [Journal Article] S1P2 receptors mediate inhibition of glioma cell migration through Rho signaling pathways independent of PTEN (2008)
  • Author(s): Malchinkhuu E, Sato K, Maehama T, Mogi C, Tomura H, Ishiuchi S, Yoshimoto Kurose H and Okajima F.
  • Journal Title: Biochem Biophys Res Commun 366 Pages: 963-968
  • Peer Reviewed
• [Journal Article] Resolvin E1 dampens airway inflammation and hyperresponsiveness in a murine model of asthma (2008)
  • Author(s): Aoki H, Hisada T, Ishizuka T, Utsugi M, Kawata T, Shimizu Y, Okajima F,Dobashi K, Mori M
  • Journal Title: Biochem Biophys Res Commun 367 Pages: 509-515
  • Peer Reviewed
• [Journal Article] Cyclooxygenase-2 expression and prostaglandin E2 production in response to acidic pH through OGR1 in a human osteoblastic cell line (2008)
  • Author(s): Tomura H, Wang JQ, Liu JP, Komachi M, Damirin A, Mogi C, Tobo M, Noch I H Tamoto K, Im DS, Sato K, Okajima F.
  • Journal Title: J Bone Miner Res 23 Pages: 1129-1139
  • Peer Reviewed
• [Journal Article] Induction of Scavenger Receptor Class B Type I is Critical for Simvastatin Enhancement of High-Density Lipoprotein-induced Anti-Inflammatory Actions in Endothelial Cells (2008)
  • Author(s): Kimura T, Mogi C, Tomura H, Kuwabara A, Im DS, Sato K, Kurose H, Murakami M, Okajima F
  • Journal Title: J Immunol 181 Pages: 7332-7340
  • Peer Reviewed
• [Journal Article] (*Corresponding author) Stimulatory Role of Lysophosphatidic Acid in COX-2 Induction by Synovial Fluid of Patients with Rheumatoid Arthritis in Fibroblast-like Synovial Cells (2008)
  • Author(s): Nochi H, Tomura H, Tobo M,Tanaka N, Sato K, Shinozaki T, Kobayashi T, Takagish I K, Ohta H, Okajima F*, Tamoto K
  • Journal Title: J Immunol 181 Pages: 5111-5119
  • Peer Reviewed
• [Journal Article] Role of lipoprotein-associated lysophospholipids in migratory activity of coronary artery smooth muscle cells (2007)
  • Author(s): Damirin A, Tomura H, Komachi M, Liu JP, Mogi C, Tobo M, Wang JQ, Kimura T, Kuwabara A, Yamazaki Y, Ohta H, Im DS, Sato K, Okajima F.
  • Journal Title: Am J Physiol Heart Circ Physiol 292 Pages: H2513-522
  • Peer Reviewed
• [Journal Article] Previously postulated "ligand-independent" signaling of GPR4 is mediatedproton-sensing mechanisms (2007)
  • Author(s): Tobo M, Tomura H, Mogi C, Wang JQ, Liu JP, Komachi M, Damirin A, Kimura K, Murata N, Kurose H, Sato K, Okajima F
  • Journal Title: Cell Signal 19 Pages: 1745-1753
  • Peer Reviewed
• [Journal Article] Critical role of ABCA1 transporter in sphingosine 1-phosphate release from astrocytes (2007)
  • Author(s): Sato K, Malchinkhuu E, Horiuchi Y, Mogi C, Tomura H, Tosaka M, Yoshimoto Y, Kuwabara A, Okajima F
  • Journal Title: J Neurochem 103 Pages: 2610-2619
  • Peer Reviewed
• [Journal Article] HDL-like lipoproteins in cerebrospinal fluid affect neural cell activity through lipoprotein-associated sphingosine 1-phosphate (2007)
  • Author(s): Sato K, Malchinkhuu E, Horiuchi Y, Mogi C, Tomura H, Tosaka M, Yoshimoto Y, Kuwabara A, Okajima F
  • Journal Title: Biochem Biophys Res Commun 359 Pages: 649-654
  • Peer Reviewed
• [Journal Article] Dioleoyl phosphatidic acid increases intracellular Ca2+ through endogenous LPA receptors in C6 glioma and L2071 fibroblasts (2007)
  • Author(s): Chang YJ, Kim YL, Lee YK, Sacket SJ, Kim K, Kim HL, Han M, Bae YS, Okajima F, Im DS
  • Journal Title: Prostaglandins Other Lipid Mediat 83 Pages: 268-276
  • Peer Reviewed
• [Presentation] 膵臓がん細胞の遊走におけるLPA受容の役体割分担 (2008)
  • Author(s): 小町麻由美、戸村秀明、岡島史和
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会BMB2007
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 20081211-15
• [Presentation] S1P isPTEN-dependent pathway in S1P2 receptor-mediated inhibitory action on glioma cell migration (2008)
  • Author(s): Malchinkhuu, E., Sato, K., and Okajima, F
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会BMB2007
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 20081211-15
• [Presentation] Acidic pH Induced Cyclooxygenase-2 Expression and Prostaglandin E2 Production through Ovarian cancer G-protein-coupled receptor 1(OGR1)in Human Osteoblasts (2008)
  • Author(s): Hideaki Tomura, Jin-peng Liu, Mayumi Komachi, Wang Ju Qiang, Masayuki Tobo Hiromi Nochi, Koichi Tamoto, Alatangaole Damirin, Koichi Sato, Chihiro Mogi, and Fumikazu Okajima
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会BMB2007
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 20081211-15
• [Presentation] グリオーマのにおける低分子量Gタンパク質とPTENの役割 (2008)
  • Author(s): 佐藤幸市、マルチンフ・エンフゾル、岡島史和
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 20081209-12
• [Presentation] Role of TDAG8 in Extracellular Acidification-induced Inhibition of Pro-inflammatory Cytokine Production in Peritoneal Macrophages (2008)
  • Author(s): Mogi, C., Tobo, M., Tomura, H., Murata, N., He, XD, Sato, K., Harada, A., Okajima, F
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 20081209-12
• [Presentation] 細胞外pHの低下に伴うヒト気管支平滑筋細胞からのインターロイキン6の産生は、OGR1受容体を介して引き起こされる (2008)
  • Author(s): 戸村秀明、一文字功、石塚全、小町麻由美、当房雅之、佐藤幸市、茂木千尋、森昌朋、岡島史和
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 20081209-12
• [Presentation] 細胞外pHの低下はOGR1受容体を介してヒト大動脈平滑筋細胞におけるシクロゲナーゼ-2の発現誘導とプロスタサイクリン産生の増加を引き起こす (2008)
  • Author(s): 劉進朋、戸村秀明、小町麻由美、当房雅之、佐藤幸市、茂木千尋、岡島史和
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 20081209-12
• [Presentation] 細胞1を介してヒト気管支平滑筋細胞からIL-6を産生させる (2008)
  • Author(s): 一文字功、石塚全、久田剛志、青木悠、森昌明、岡島史和
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 20081127-29
• [Presentation] スフィンゴシン1-リン酸の細胞外放出とABCA1トランスポーター (2008)
  • Author(s): 岡島史和、木村孝穂、戸村秀明、佐藤幸市
  • Organizer: 第50回脂質生化学会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
  • Year and Date: 20080605-06
• [Presentation] ABCA1トランスポーターによる血漿リポ蛋白質産生とスフィンゴシン1-リン酸の動態調節 (2008)
  • Author(s): 佐藤幸市、マルチンフ エンフゾル、岡島史和
  • Organizer: 第50回脂質生化学会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
  • Year and Date: 20080605-06
• [Presentation] 膵臓がん細胞の遊走におけるリゾホスファチジン酸受容体サブタイプの役割 (2008)
  • Author(s): 小町麻由美、戸村秀明、岡島史和
  • Organizer: 第50回脂質生化学会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
  • Year and Date: 20080605-06
• [Presentation] ヒト骨芽細胞における細胞外pHの低下に伴うCOX-2発現誘導・PGE2産生とOGR1受容体の関連解析 (2008)
  • Author(s): 戸村秀明、劉進朋、当房雅之、Doon-Soon Im、茂木千尋、岡島史和
  • Organizer: 第50回脂質生化学会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
  • Year and Date: 20080605-06
• [Presentation] Enhacement of High-densityLipoprotein-induced Anti-Inflammatory Actions in Endothelial Cells by Simvastatin-induced expression of Scavenger Receptor Class B Type I (2008)
  • Author(s): Kimura, T., Okajima, F., and Murakami, M.
  • Organizer: The 72^<st> Annual Scientific Meeting of the Japansese Circulation Society
  • Place of Presentation: Fukuoka
  • Year and Date: 20080328-30
• [Presentation] Roles of lipoproteins as carriers of lysolipids (2007)
  • Author(s): Oka jima, F
  • Organizer: 2007 FASEB Summer Research Conferences
  • Place of Presentation: Tucson, Arizona
  • Year and Date: 20070609-14
• [Presentation] ヒト臍帯静脈内皮細胞における高密度リポ蛋白質HDLの接着分子発現抑制作用~HDL受容体SR-BIとS1P受容体の役割 (2007)
  • Author(s): 木村孝穂、戸村秀明、桑原敦志、茂木千尋、佐藤幸市、村上正巳、岡島史和
  • Organizer: 第49回日本脂質生化学会
  • Place of Presentation: 北海道大学、学術交流会館(札幌)
  • Year and Date: 20070605-06
• [Presentation] Spingosine 1-phosphatisoproterenol negatively but differently regulate LPA-induced migration in human glioma cells (2007)
  • Author(s): Malchinkhuu Enkhzol, 佐藤幸市、岡島史和
  • Organizer: 第49回日本脂質生化学会
  • Place of Presentation: 北海道大学、学術交流会館(札幌)
  • Year and Date: 20070605-06
• [Presentation] Roles of lipoproteins as carriers of sphingosine 1-phophate (2007)
  • Author(s): Okajima, F
  • Organizer: Pusan National University College of Medicine
  • Place of Presentation: Pusan, Korea
  • Year and Date: 2007-10-30
• [Presentation] Roles of lipoproteins as carriers of sphingosine 1-phosphate (2007)
  • Author(s): Okajima, F
  • Organizer: College of Pharmacy, Pusan National University
  • Place of Presentation: Pusan, Korea
  • Year and Date: 2007-10-29
• [Presentation] リポ蛋白質作用におけるスフィンゴシン1-リン酸受容体とスカベンジャー受容体の役割 (2007)
  • Author(s): 岡島史和
  • Organizer: 第6回生体機能研究会
  • Place of Presentation: 箱根プリンスホテル
  • Year and Date: 2007-09-22
• [Remarks]
  • URL: http://imcr.showa.gunma-u.ac.jo/lab/signal/index.html