Molecular mechanism of accumulation and rearrangement of antibiotic resistance gene in bacteria.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390127
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: ARAKAWA Yoshichika National Institute of Infectious Diseases, 細菌第二部, 部長 (10212622)
• Co-Investigator(Kenkyū-buntansha): SHIBAYAMA Keigo 国立感染症研究所, 細菌第二部, 室長 (50283437) ; MORI Shigetarou 国立感染症研究所, 細菌第二部, 主任研究官 (60425676) ; YAMANE Kunikazu 国立感染症研究所, 細菌第二部, 主任研究官 (00356247) ; SUZUKI Satowa 国立感染症研究所, 細菌第二部, 主任研究官 (30373400) ; KIMURA Kouji 国立感染症研究所, 細菌第二部, 主任研究官 (50425675) ; WACHINO Jun-ichi 国立感染症研究所, 細菌第二部, 研究員 (00535651) ; MATSUI Mari 国立感染症研究所, 細菌第二部, 研究員 (50555761)
• Project Period (FY): 2007 – 2009
• Keywords: 薬剤耐性菌 / 遺伝子転位機構 / クラス3インテグロン

Research Abstract

We determined the genetic structure of class 3 integrons in Serratia marcescens and Pseudomonas putida clinical isolates. The nucleotide regions including class 3 integron were flanked by 25bp inverted repeats and co-located with the elements such as TniA, TniB, TniQ, and TniC, which are thought to be required for the transposition of class 3 integron. Recombinant IntI3 protein was expressed in Escherichia coli, purified using column chromatography, and crystallized by hanging drop vapor diffusion method. However, we could not obtain a good X-ray diffraction data from the crystals. In Acinetobacter baumannii clinical isolates, ISAbaI was located upstream of bla_<OXA-51-like>, and apperared to provide a promoter activity for expression of adjacent bla_<OXA-51-like>.