The development of noninvasive and economical method to evaluate the bone quality using the measurement of serum levels of osteocyte-specific-proteins

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390393
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Osaka University
• Principal Investigator: HASHIMOTO Jun Osaka University, 医学系研究科, 准教授 (40237938)
• Co-Investigator(Kenkyū-buntansha): YOSHIKAWA Hideki 大阪大学, 医学系研究科, 教授 (60191558) ; MORIYAMA Toshiki 大阪大学, 保健センター, 教授 (30283815) ; MYOUI Akira 大阪大学, 医学部附属病院, 准教授 (10263261) ; NAKAMURA Norimasa 大阪大学, 臨床医工学融合研究教育センター, 招聘教授 (50273719)
• Project Period (FY): 2007 – 2009
• Keywords: MEPE / 骨細胞 / 骨質 / 骨強度 / 酸素分圧 / 椎体骨折

Research Abstract

It is revealed by our preliminary analysis that serum level of matrix extracellular phosphoglycoprotein (MEPE) that is one of osteocyte-specific-proteins is correlated with the multiplicity of osteoporotic vertebral fracture. However, the amino sequence analysis of MEPE derived fragment present in serum using monoclonal antibodies against MEPE revealed that mAb also bind with the unknown peptide derived from some protein other than MEPE. Identification of this foreign peptide is unfortunately too difficult due to its small amount and the cross reactivity itself in mAb, so clinical application of ELISA using mAb against MEPE has not been established. However, our in vitro study confirmed that the possible importance of the measurement of osteocyte-specific protein and showed the next target protein for development of clinically applicable plasma concentration measurement.

Research Products

• [Journal Article] High oxygen tension prolongs the survival of osteoclast precursors via macrophage colony-stimulating factor Bone. (2009)
  • Author(s): Yamasaki N, Tsuboi H, Hirao M, Nampei A, Yoshikawa H, Hashimoto J
  • Journal Title: Bone 44 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Alendronate Treatment Promotes Bone Formation with a Less Anisotropic Microstructure during Intramembranous Ossification in Rats (2008)
  • Author(s): Kashii M, Hashimoto J, Nakano T, Umakoshi Y, Yoshikawa H
  • Journal Title: J Bone Miner Metab 26(1) Pages: 24-33
  • Peer Reviewed
• [Journal Article] Response of serum carboxylated and undercarboxylated osteocalcin to alendronate monotherapy and combined therapy with vitamin K2 in postmenopausal women (2008)
  • Author(s): Hirao M, Hashimoto J, Ando W, Ono T, Yoshikawa H
  • Journal Title: J Bone Miner Metab 26(3) Pages: 260-4
  • Peer Reviewed
• [Journal Article] In Vitro Generation of a Scaffold-Free Tissue-Engineered Construct (TEC) Derived from Human Synovial Mesenchymal Stem Cells: Biological and Mechanical Properties, and Further Chondrogenic Potential. (2008)
  • Author(s): Ando W, Tateishi K, Katakai D, Hart DA, Higuchi C, Nakata K, Hashimoto J, Fujie H, Shino K, Yoshikawa H, Nakamuara N
  • Journal Title: Tissue Eng Part A 14(12) Pages: 2041-9
  • Peer Reviewed
• [Journal Article] Oxygen tension is an important mediator of the transformation of osteoblasts to osteocytes. (2007)
  • Author(s): Hirao M, Hashimoto J, Yamasaki N, Ando W, Tsuboi H, Myoui A, Yoshikawa H
  • Journal Title: J Bone Miner Metab 25(5) Pages: 266-76
  • Peer Reviewed
• [Journal Article] Ito T Risedronate, an effective treatment for glucocorticoid-induced bone loss in CKD patients with or without concomitant active vitamin (2007)
  • Author(s): Fujii N, Hamano T, Mikami S, Nagasawa Y, Isaka Y, Moriyama T, Horio M, Imai E, Hori M
  • Journal Title: D Nephrol Dial 22(6)(Transplant) Pages: 1601-1607
  • Peer Reviewed
• [Journal Article] Parthenolide, a natural inhibitor of nuclear factor-kappab, inhibits lung colonization of murine osteosarcoma cells. (2007)
  • Author(s): Kishida Y, Yoshikawa H, Myoui A
  • Journal Title: Clin Cancer Res 13 Pages: 59-67
  • Peer Reviewed