The role of PPAR in metabolic syndrome with novel genetically modified rabbits

2009 Fiscal Year Final Research Report

• Project/Area Number: 19500362
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Saga University
• Principal Investigator: KITAJIMA Shuji Saga University, 総合分析実験センター, 准教授 (70284643)
• Co-Investigator(Kenkyū-buntansha): FAN Jianglin 山梨大学, 医学工学総合研究部, 教授 (60272192) ; NISHIJIMA Kazutoshi 佐賀大学, 総合分析実験センター, 助教 (70435874)
• Project Period (FY): 2007 – 2009
• Keywords: 遺伝子組換え / ウサギ / 肥満 / 病態モデル / PPAR

Research Abstract

We generated transgenic (Tg) rabbits as a novel human disease model for metabolic syndrome. PPAR-gamma has been believed to be associated with pathologic status of it. Tg rabbits was designed to specifically overexpress human PPAR-gamma in their adipose tissue. As the results, we confirmed specific expression of mRNA of transgene in the adipose tissue of Tg rabbits, however, we could not find phenotypic differences (pathologic status) between Tg rabbits and wild type one. It is thought that more detailed further examination such as manifestation protein activity is required in future.

Research Products

• [Journal Article] Human C-reactive protein does not promote atherosclerosis in transgenic rabbits. (2009)
  • Author(s): Koike, T., Kitajima, S., Yu, Y., Nishijima, K., Zhang, J., Ozaki, Y., Morimoto, M., Watanabe, T., Bhakdi, S., Asada, Y., Chen, Y.E., Fan, J.
  • Journal Title: Circulation 120 Pages: 2088-2094
• [Journal Article] Expression of human apoAII in transgenic rabbits leads to dyslipidemia: a new model for combined hyperlipidemia. (2009)
  • Author(s): Koike, T., Kitajima, S., Yu, Y., Li, Y., Nishijima, K., Liu, E., Sun, H., Ahmed Bilal Waqar, A.B., Shibata, N., Inoue, T., Wang, Y., Zhang, B, Kobayashi, J, Morimoto, M., Saku, K., Watanabe, T., Fan, J.
  • Journal Title: Arterioscler. Thromb. Vasc. Biol. 29 Pages: 2047-2053
• [Journal Article] Temporal and quantitative analysis of expression of metalloproteinase (MMPs) and their endogenous inhibitors in atherosclerotic lesions. (2008)
  • Author(s): Yu, Y., Koike, T., Kitajima, S., Liu, E., Morimoto, M., Shiomi, M., Hatakeyama, K., Asada, Y., Wang, K-Y., Sasaguri, Y., Watanabe, T., Fan, J.
  • Journal Title: Histol. Histopathol. 23 Pages: 1503-1516
• [Journal Article] Matrix metalloproteinase 12 is a factor that accelerates the initiation of atherosclerosis and stimulates the progression of fatty streaks to fibrous plaques in transgenic rabbits. (2008)
  • Author(s): Yamada, S., Wang, K-Y., Tanimoto, A., Fan, J., Shimajiri, S., Kitajima, S., Morimoto, M., Tsutsui, M., Watanabe, T., Yasumoto, K., Sasaguri, Y.
  • Journal Title: Am. J. Pathol. 172 Pages: 1419-1429
• [Journal Article] 凍結精子による遺伝子組換えウサギの系統保存 (2008)
  • Author(s): 北嶋修司, 森本正敏
  • Journal Title: 関西実験動物研究会会報 30 Pages: 16-20
• [Journal Article] Increased expression of vascular endothelial growth factor in kidney leads to progressive impairment of glomerular functions. (2007)
  • Author(s): Liu, E., Morimoto, M., Kitajima, S., Yu, Y., Koike, T., Shiiki, H., Nagata, M., Watanabe, T., Fan, J.
  • Journal Title: J. Am. Soc. Nephrol. 18 Pages: 2094-2104
• [Journal Article] Lp(a) enhances coronary atherosclerosis in transgenic Watanabe heritable hyperlipidemic rabbits. (2007)
  • Author(s): Kitajima, S., Jin, Y., Koike, T., Yu, Y., Liu, E., Morimoto, M., Shiomi, M., Marcovina, S., Watanabe, N. Fan, J.
  • Journal Title: Atherosclerosis 193 Pages: 269-276
• [Journal Article] Numbers of publications related to laboratory animals. (2007)
  • Author(s): Zhao, S., Liu, E., Chu, Y., Zheng, H., Kitajima, S., Morimoto, M.
  • Journal Title: Scand. J. Lab. Anim. Sci. 34 Pages: 81-86
• [Journal Article] Re-establishment of Complement C6-deficeint Rabbit Colony by Cryopreserved Sperm Transported from Abroad. (2007)
  • Author(s): Liu, E., Kitajima, S., Wiese, E., Reifenberg, K., Morimoto, M., Watanabe, T., Fan, J.
  • Journal Title: Exp. Anim. 55(2) Pages: 167-171
• [Journal Article] ヒト疾患モデルとしての遺伝子組換えウサギの開発とその系統保存. (2007)
  • Author(s): 北嶋修司, 詫广茂信, 友田清光, 森本正敏
  • Journal Title: 九州実験動物雑誌 23 Pages: 31-45
• [Presentation] ウサギの血清分離方法の検討. (2009)
  • Author(s): 漢那宗士, 西島和俊, 常吉梨沙, 森本正敏, 北嶋修司
  • Organizer: 第43回日本実験動物技術者協会総会
  • Place of Presentation: 新潟
  • Year and Date: 20091009-20091010
• [Presentation] Transgenic rabbits expressing Human C-reactive protein. (2009)
  • Author(s): Koike, T., Kitajima, S., Yu, Y., Nishijima, K., Zhang, J., Ozaki, Y., Morimoto, M., Watanabe, T., Asada, Y., Chen, E., Fan, J.
  • Organizer: 第41回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 下関
  • Year and Date: 20090717-20090718
• [Presentation] Immunoreactivity of neuropeptides in hypothalamus of the transgenic rabbits using immunohisotchemical staining. (2009)
  • Author(s): Morimoto, M., Kitajima, S., Nishijima, K., Koike, T., Fan, J.
  • Organizer: 3rd International Meeting on Rabbit Biotechnology
  • Place of Presentation: Xian, China
  • Year and Date: 20090604-20090605
• [Presentation] Comparison of two rabbit semen cryopreservation protocol-a pilot study. (2009)
  • Author(s): Szikra, D., Nagy, S., Bernder, B., Hiripi, L., Kitajima, S., Pribenszky, C., Bosze, Z.
  • Organizer: 3rd International Meeting on Rabbit Biotechnology
  • Place of Presentation: Xian, China
  • Year and Date: 20090604-20090605
• [Presentation] Transgenic rabbits expressing human C-reactive protein. (2009)
  • Author(s): Koike, T., Yu, Y., Kitajima, S., Zhang, J., Bhakdi, S., Chen, E.Y., Fan, J.
  • Organizer: 3rd International Meeting on Rabbit Biotechnology
  • Place of Presentation: Xian, China
  • Year and Date: 20090604-20090605
• [Presentation] Technology suitable for rabbit semen conservation. (2009)
  • Author(s): Kitajima, S., Maeda, T., Liu, E., Nishijima, K., Morimoto, M., Watanabe, T.
  • Organizer: 3rd International Meeting on Rabbit Biotechnology
  • Place of Presentation: Xian, China
  • Year and Date: 20090604-20090605
• [Presentation] ウサギ採卵成績に影響を及ぼす要因の検討:過去5年間の採卵成績の解析. (2009)
  • Author(s): 北嶋修司, 西島和俊, 森本正敏, 渡辺照男, 範江林
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 埼玉
  • Year and Date: 20090514-20090516
• [Presentation] Transgenic Rabbits Expressing human C-reactive Protein.Arteriosclerosis (2009)
  • Author(s): Koike, T., Fan, J., Yu, Y., Kitajima, S., Zhang., J., Bhakdi., S., Chen, E.Y.
  • Organizer: Thrombosis and Vascular Biology Annual Conference 2009
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20090429-20090501
• [Presentation] 細胞外基質分解酵素MMP-12は、初期動脈硬化巣の形成から進展に重要な促進因子である. (2008)
  • Author(s): 山田壮亮, 王克〓, 谷本昭英, 範江林, 島尻正平, 北嶋修司, 森本正敏, 渡邊照男, 笹栗靖之
  • Organizer: 第3回フォーラム「医療に貢献する実験用ウサギの新しい展開」
  • Place of Presentation: 神戸
  • Year and Date: 2008-07-26
• [Presentation] 動脈硬化病変のC反応性蛋白-その由来と病態生理学的重要性. (2007)
  • Author(s): 小池智也, 北嶋修司, 森本正敏, 塩見雅志, 渡辺照男, 浅田祐士郎, 畠山金太, 範江林
  • Organizer: 第24回日本疾患モデル動物学会総会
  • Place of Presentation: 筑波
  • Year and Date: 20070831-20070901
• [Presentation] Rabbit semen conservation. (2007)
  • Author(s): Kitajima, S.
  • Organizer: 2nd International Meeting on Rabbit Biotechnology
  • Place of Presentation: Paris, France
  • Year and Date: 20070614-20070615
• [Presentation] 凍結融解後のウサギ精子受精能に及ぼす冷却速度の影響について (2007)
  • Author(s): 前田達弘, 劉恩岐, 詫广茂信, 北嶋修司, 森本正敏
  • Organizer: 第25回九州実験動物研究会
  • Place of Presentation: 熊本
  • Year and Date: 2007-11-10
• [Presentation] 凍結精子による遺伝子組換えウサギの系統保存(招待講演).「トランスレーショナルリサーチにおけるウサギの重要性」 (2007)
  • Author(s): 北嶋修司
  • Organizer: 第95回関西実験動物研究会
  • Place of Presentation: 神戸大学
  • Year and Date: 2007-09-14
• [Book] Improvement of rabbit production. Rabbit Biotechnology: rabbit genomics, transgenesis, cloning and models. Houdebine, L.-M. and Fan, J. (2009)
  • Author(s): Kitajima, S.
  • Total Pages: 3-12
  • Publisher: Springer
• [Book] Rabbit transgenesis. Rabbit Biotechnology: rabbit genomics, transgenesis, cloning and models. Houdebine, L.-M. and Fan, J. (2009)
  • Author(s): Kitajima, S., Liu, E., Fan, J.
  • Total Pages: 37-48
  • Publisher: Springer Heidelberg
• [Remarks] ホームページ等
  • URL: http://www.animal.med.saga-u.ac.jp/