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2010 Fiscal Year Final Research Report

Feasible study of a phenome analysis using chemicals and a high throughput screening system in Arabidopsis

Research Project

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Project/Area Number 19510206
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied genomics
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KUROMORI Takashi  The Institute of Physical and Chemical Research, 機能開発研究グループ, 研究員 (80332295)

Co-Investigator(Renkei-kenkyūsha) NOUTOSHI Yoshiteru  独立行政法人理化学研究所, 植物免疫研究チーム, 客員研究員 (70332278)
MYOUGA Fumiyoshi  独立行政法人理化学研究所, 機能開発研究グループ, 研究員 (10342859)
Project Period (FY) 2007 – 2010
Keywords植物 / シロイヌナズナ / ゲノム / フェノーム
Research Abstract

Conditional phenotypes are mutant phenotypes which can be detected under some stress treatment or environmental conditions. We developed a high throughput assay system to detect the conditional phenotypes for many tagging lines quickly using multi-titer plates. According to image scanning, we could calculate the green parts of each well that is indicating the growth status. By this detection system, we could find a novel ABA hypersensitive mutant.

  • Research Products

    (36 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (12 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana.2011

    • Author(s)
      Kuromori T
    • Journal Title

      Mol.Biol.Evol. 28

      Pages: 377-382

    • Peer Reviewed
  • [Journal Article] ABA transport factors found in Arabidopsis ABC transporters.2010

    • Author(s)
      Kuromori T, Shinozaki K.
    • Journal Title

      Plant Signal Behav. 5

      Pages: 1124-1126

    • Peer Reviewed
  • [Journal Article] Molecular basis of the core regulatory network in abscisic acid responses: sensing, signaling, and transport.2010

    • Author(s)
      Umezawa T, Nakashima K, Miyakawa T, Kuromori T, Tanokura M, Shinozak K, Yamaguchi-Shinozaki K.
    • Journal Title

      Plant Cell Physiol. 51

      Pages: 1821-1839

    • Peer Reviewed
  • [Journal Article] Phenome analysis of root development in Arabidopsis.2010

    • Author(s)
      Yuguchi M, Yokouchi T, Tominaga-Wada R, Kuromori T, Shinozaki K, Okada K, Wada T.
    • Journal Title

      Plant Biotech. 27

      Pages: 345-347

    • Peer Reviewed
  • [Journal Article] Toward genome-wide metabolotyping and elucidation of metabolicsystem : metabolic profiling of large-scale bioresources.2010

    • Author(s)
      Kuromori T
    • Journal Title

      J.Plant Res. 123

      Pages: 291-298

    • Peer Reviewed
  • [Journal Article] ABC transporter AtABCG25 is involved in abscisic acid transport and responses.2010

    • Author(s)
      Kuromori T, Miyaji T, Yabuuchi H, Shimizu H, Sugimoto E, Kamiya A, Moriyama Y, Shinozaki K.
    • Journal Title

      Proc.Natl Acad.Sci.USA 107

      Pages: 2361-2366

    • Peer Reviewed
  • [Journal Article] T-DNA-tagged homozygous lines for nuclear-encoded chloroplast proteins and their systematic phenotype analysis.2010

    • Author(s)
      Kuromori T
    • Journal Title

      Plant J. 61

      Pages: 529-542

    • Peer Reviewed
  • [Journal Article] Increased expression and protein divergence in duplicate genes is associated with morphological diversification.2009

    • Author(s)
      Hanada K, Kuromori T, Myoga F, Toyoda T, Shinozaki K
    • Journal Title

      PLoS Genet. 5

      Pages: e1000781

    • Peer Reviewed
  • [Journal Article] Evolutionary persistence of functional compensation by duplicate genes in Arabidopsis.2009

    • Author(s)
      Hanada K, Kuromori T, Myoga F, Toyoda T, Li WH, Shinozaki K
    • Journal Title

      Gen.Biol.Evol. 2009

      Pages: 409-414

    • Peer Reviewed
  • [Journal Article] Phenome analysis in plant species using loss-of-function and gain-of-function mutants.2009

    • Author(s)
      Kuromori T, Takahashi S, Kondou Y, Shinozaki K, Matsui M
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 1215-1231

    • Peer Reviewed
  • [Journal Article] PosMed-plus : an intelligent search engine that inferentially integrates cross-species information resources for molecular breeding of plants.2009

    • Author(s)
      Kuromori T
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 1249-1259

    • Peer Reviewed
  • [Journal Article] MS/MS spectral tag (MS2T)-based annotation of non-targeted profile of plant secondary metabolites.2009

    • Author(s)
      Matsuda F, Yonekura-S.K, Niida R, Kuromori T, Shinozaki K, Saito K
    • Journal Title

      Plant J. 57

      Pages: 555-577

    • Peer Reviewed
  • [Journal Article] Homologous chromosome pairing is completed in crossover defective atzip4 mutant.2008

    • Author(s)
      Kuromori T, Azumi Y, Hayakawa S, Kamiya A, Imura Y, Wada T, Shinozaki K
    • Journal Title

      Biochem.Biophys.Res.Commun. 370

      Pages: 98-103

    • Peer Reviewed
  • [Journal Article] The novel glycerophosphoryl diester phosphodiesterase-like proteins SHV3 and SVLs are pivotal players in cell wall organization.2008

    • Author(s)
      Hayashi S, Ishii T, Matsunaga T, Tominaga R, Kuromori T, Wada T, Shinozaki K, Hirayama T
    • Journal Title

      Plant Cell Physiol. 49

      Pages: 1522-1535

    • Peer Reviewed
  • [Journal Article] A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis.2008

    • Author(s)
      Kuromori T
    • Journal Title

      Plant Cell. 20

      Pages: 3148-3162

    • Peer Reviewed
  • [Journal Article] Cytological and Biochemical Analysis of COF1, an Arabidopsis Mutant of an ABC Transporter Gene.2007

    • Author(s)
      Ukitsu H, Kuromori T, Toyooka K, Goto Y, Matsuoka K, Sakuradani E, Shimizu S, Kamiya A, Imura Y, Yuguchi M, Wada T, Hirayama T, Shinozaki K
    • Journal Title

      Plant Cell Physiol. 48

      Pages: 1524-1533

    • Peer Reviewed
  • [Journal Article] Top-down phenomics of Arabidopsis thaliana - metabolic profiling by one- and two-dimensional nuclear magnetic resonance SPECTROSCOPY and transcriptome analysis of albino mutants.2007

    • Author(s)
      Tian C, Chikayama E, Tsuboi Y, Kuromori T, Shinozaki K, Kikuchi J, Hirayama T
    • Journal Title

      J.Biol.Chem. 282

      Pages: 18532-18541

    • Peer Reviewed
  • [Journal Article] Multiple loss-of-function of Arabidopsis gibberellin receptor AtGID1s completely shuts down a gibberellin signal.2007

    • Author(s)
      Kuromori T
    • Journal Title

      Plant J. 50

      Pages: 958-966

    • Peer Reviewed
  • [Presentation] Regulatory gene network in early responses to drought stress.2011

    • Author(s)
      Shinozaki K
    • Organizer
      Plant Abiotic Stress Tolerance Mechanisms, Water and Global Agriculture (Keystone Symposia)
    • Place of Presentation
      Colorado, USA
    • Year and Date
      20110117-20110121
  • [Presentation] Development of a database focusing phenome of Arabidopsis thaliana on RIKEN SciNeS.2010

    • Author(s)
      Doi K
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-20101210
  • [Presentation] ABA transport and the functional factors found in Arabidopsis ABC transporters.2010

    • Author(s)
      Kuromori T
    • Organizer
      From Plant Biology to Crop Biotechnology (2010 Cold Spring Harbor Asia Conference)
    • Place of Presentation
      Suzhou, China
    • Year and Date
      20101025-20101029
  • [Presentation] AtABCG25 is an ABC transporter involved in abscisic acid transport and response.2010

    • Author(s)
      Shinozaki K
    • Organizer
      Plant Biology 2010
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20100731-20100804
  • [Presentation] Arabidopsis AtABCG25 is an ABC transporter involved in ABA transport and responses.2010

    • Author(s)
      Kuromori T
    • Organizer
      20th International Conference on Plant Growth Substances (IPGSA)
    • Place of Presentation
      Tarragona, Spain
    • Year and Date
      20100628-20100702
  • [Presentation] ABC transporter AtABCG25 involved in ABA transport and responses.2010

    • Author(s)
      Kuromori T
    • Organizer
      International Conference on Arabidopsis Research (ICAR 2010)
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20100606-20100610
  • [Presentation] RIKEN SciNes : A semantic-web database integrating phenome of Arabidopsis thaliana.2010

    • Author(s)
      Doi K
    • Organizer
      International Conference on Arabidopsis Research (ICAR 2010)
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20100606-20100610
  • [Presentation] セマンティックウェブを利用したシロイヌナズナの表現型および関連情報の統合の試み2009

    • Author(s)
      Doi K
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20091209-20091212
  • [Presentation] PosMed-plus : An information system making the utmost use of Arabidopsis knowledge to assist molecular breeding of crops.2009

    • Author(s)
      Makita Y
    • Organizer
      International Conference on Arabidopsis Research (ICAR 2009)
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      20090630-20090704
  • [Presentation] Systematic phenotype analysis of Arabidopsis Ds-tagged mutants to unravel gene functions in abitoic stress response as well as growth and development.2008

    • Author(s)
      Kuromori T
    • Organizer
      International Symposium on Induced Mutations in Plants (ISIM)
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      20080812-20080815
  • [Presentation] Cytological and biochemical analysis of Arabidopsis mutants of ABC transporter gene COF1/WBC11.2007

    • Author(s)
      Kuromori T
    • Organizer
      BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会)
    • Place of Presentation
      横浜
    • Year and Date
      20071211-20071215
  • [Presentation] A mutant-based phenome analysis and collection of mutant phenotype information.2007

    • Author(s)
      Kuromori T
    • Organizer
      International Conference on Arabidopsis Research (ICAR 2010)
    • Place of Presentation
      Beijing, China
    • Year and Date
      20070620-20070623
  • [Book] Dstransposon mutant lines for saturation mutagenesis of the Arabidopsis genome.(KGaA, Weinheim. Molecular Plant Biology Handbook Series, The Handbook of Plant Mutation Screening : Mining of Natural and Induced Alleles.)(Chapter 2)2010

    • Author(s)
      Kuromori T, Hirayama T
    • Total Pages
      17-30
    • Publisher
      WILEY-VCH Verlag GmbH & Co.
  • [Book] Induced Plant Mutations in the Genomics Era.(Systematic phenotype analysis of Arabidopsis Ds-tagged mutants to unravel gene functions in abiotic stress response as well as growth and development.)2009

    • Author(s)
      Kuromori T, Shinozaki K
    • Total Pages
      131-133
    • Publisher
      Food and Agriculture Organization of the United Nations, Rome.
  • [Remarks] プレスリリース, 2010年1月19日, 乾燥耐性を誘起する植物ホルモン「アブシジン酸」の輸送因子を発見

    • URL

      http://www.riken.jp/r-world/info/release/press/2010/100119/index.html

  • [Remarks] ホームページ

    • URL

      http://labs.psc.riken.jp/gdrg/index.html#g_project2

  • [Patent(Industrial Property Rights)] アブシジン酸輸送タンパク質を過剰発現する植物及びその作出方法2010

    • Inventor(s)
      黒森崇、篠崎一雄
    • Industrial Property Rights Holder
      (独)理化学研究所
    • Industrial Property Number
      特願,PCT/JP2010/073664
    • Filing Date
      2010-12-21
    • Overseas
  • [Patent(Industrial Property Rights)] アブシジン酸輸送タンパク質を過剰発現する植物及びその作出方法2009

    • Inventor(s)
      黒森崇、篠崎一雄
    • Industrial Property Rights Holder
      (独)理化学研究所
    • Industrial Property Number
      特願,2009-289457
    • Filing Date
      2009-12-21

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Published: 2012-01-26   Modified: 2016-04-21  

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