2009 Fiscal Year Final Research Report
Genetic study on overexpression mechanisms of cytochrome P450 in insecticide resistant insect
| Project/Area Number |
19580062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
TOMITA Takashi National Institute of Infectious Diseases, 昆虫医科学部, 室長 (20180169)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Shinji 国立感染症研究所, 昆虫医科学部, 主任研究官 (80332360)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 殺虫剤抵抗性 / シトクロムP450 / 遺伝子過剰発現 / 遺伝子重複 / シス作用性調節変異 / ネッタイイエカ / ピレスロイド |
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| Research Abstract |
A P450 gene Cyp9m10 is about 260-fold overexpressing in a pyrethroid-resistant strain of Culex quinquefasciatus compared to a susceptible strain. a region of approximately 100kb in length including the Cyp9m10 locus was specifically duplicated in the resistant strain. The two duplicated Cyp9m10 copies shared a completely identical sequence within the transcribed region and the flanking region up to the breakpoint located 1.1kb upstream of the transcriptional start site. A Miniature Inverted-repeat Transposable Element (MITE)-like element was specifically inserted 0.2kb upstream of both Cyp9m10 copies in the resistant strain. We obtained direct evidence that the Cyp9m10 overexpression is caused by a cis-acting mutation. In backcross experiment, a haplotype containing the two duplicated Cyp9m10 copies was strongly associated with the pyrethroid resistance.
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