2009 Fiscal Year Final Research Report
Functional analysis of magnesium transport proteins in plant chloroplasts
| Project/Area Number |
19580126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
ISHIJIMA Sumio Kyoto Prefectural University, 大学院・生命環境科学研究科, 准教授 (70184520)
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| Project Period (FY) |
2007 – 2009
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| Keywords | マグネシウムイオン / 膜輸送タンパク質 / 葉緑体 / リポソーム / シロイヌナズナ |
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| Research Abstract |
We have analyzed the Mg^<2+> transport function of plant chloroplast protein molecules in this study. Plant proteins were expressed in Escherichia coli, and purified using an appropriate detergent. Isolated AtMRS2-10 from Arabidopsis thaliana was reconstituted into liposomes, and Mg^<2+> transport activity into liposomes was assayed using a fluorescent dye and with atomic absorption spectroscopy. This system allows first measurement of Mg^<2+> transport activity of plant protein.
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Research Products
(8 results)
