2009 Fiscal Year Final Research Report
Functional analysis of magnesium transport proteins in plant chloroplasts
| Project/Area Number |
19580126
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Kyoto Prefectural University |
|---|
Principal Investigator |
ISHIJIMA Sumio Kyoto Prefectural University, 大学院・生命環境科学研究科, 准教授 (70184520)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | マグネシウムイオン / 膜輸送タンパク質 / 葉緑体 / リポソーム / シロイヌナズナ |
|---|
| Research Abstract |
We have analyzed the Mg^<2+> transport function of plant chloroplast protein molecules in this study. Plant proteins were expressed in Escherichia coli, and purified using an appropriate detergent. Isolated AtMRS2-10 from Arabidopsis thaliana was reconstituted into liposomes, and Mg^<2+> transport activity into liposomes was assayed using a fluorescent dye and with atomic absorption spectroscopy. This system allows first measurement of Mg^<2+> transport activity of plant protein.
|
