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2008 Fiscal Year Final Research Report

Development of effective transformation methods for marine phytoplankton using Agrobacterium

Research Project

  • PDF
Project/Area Number 19580206
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionKochi University

Principal Investigator

ADACHI Masao  Kochi University, 教育研究部・自然科学系, 教授 (70274363)

Co-Investigator(Kenkyū-buntansha) 大西 浩平  高知大学, 教育研究部・総合科学系, 教授 (50211800)
Project Period (FY) 2007 – 2009
Keywordsプランクトン / 遺伝子導入
Research Abstract

本研究では、まず海産プランクトンに対し、様々な条件の下でアグロバクテリウムを感染させ、その遺伝子導入を試みたが、形質転換体を得ることは出来なかった。そこで、海産藻類感染性ウイルスに由来するプロモーターを新たに用い、これを組み込んだ形質転換用プラスミドを構築した。本プラスミドを用いることにより、中心目珪藻種や羽状目珪藻種に対し、目レベルを超えて形質転換が可能であることが判明した。本結果は、海産藻類ウイルス由来のプロモーターの形質転換系への適用事例として、世界初の成果である。

  • Research Products

    (10 results)

All 2009 2008

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] High efficiency transformation of the diatom Phaeodactylum tricornutum with a promoter from the diatom Cylindrotheca fusiformis2009

    • Author(s)
      Miyagawa A., Okami T., Kira N., Yamaguchi H., Ohnishi K. and Adachi M.
    • Journal Title

      Phycological Research 57

      Pages: 142-146

    • Peer Reviewed
  • [Journal Article] The pleiotropic regulator PhcA negatively controls expression of the first component of a regulatory cascade necessary for Ralstonia solanacearum hrp regulon induction2009

    • Author(s)
      Yoshimochi T., Hikichi Y., Kiba A. and Ohnishi K.
    • Journal Title

      J.Bacteriol (in press)

    • Peer Reviewed
  • [Journal Article] Occurrence of toxic Dinophysis acuminata (Dinophyceae) in Uranouchi Inlet, Japan2008

    • Author(s)
      Adachi M., Okamoto N., Matsubara M., Nishijima T. and Suzuki T.
    • Journal Title

      Fisheries Science 74

      Pages: 1351-1321

    • Peer Reviewed
  • [Journal Article] The hrp genes of Pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce2008

    • Author(s)
      Hojo H., Koyanagi M., Tanaka M., Kajihara S., Ohnishi K., Kiba A. and Hikichi Y.
    • Journal Title

      Microbiology 154

      Pages: 2920-2928

    • Peer Reviewed
  • [Journal Article] Crystal structure of pyridoxamine-pyruvate amino-transferase from Mesorhizobium loti MAFF3030992008

    • Author(s)
      Yoshikane Y., Yokochi N., Yamasaki M., Mizutani K., Ohnishi K., Mikami B., Hayashi H. and Yagi T.
    • Journal Title

      J.Biol. Chem. 283

      Pages: 1120-1127

    • Peer Reviewed
  • [Presentation] Development of marine diatom transformation system using viral promoter2009

    • Author(s)
      Arisa Miyagawa, Yuji Tomaru, Keizo Nagasaki, Haruo Yamaguchi and Masao Adachi
    • Organizer
      SCOR Viral Ecology Meeting, Poster Presentation
    • Place of Presentation
      Newark, USA
    • Year and Date
      20090517-21
  • [Presentation] 藻類感染性ウイルスプロモーターを用いた海産珪藻の形質転換系の開発2009

    • Author(s)
      宮川亜利沙・外丸祐司・長崎慶三・山口晴生・足立真佐雄
    • Organizer
      第12回マリンバイオテクノロジー学会
    • Place of Presentation
      早稲田大学大久保キャンパス
    • Year and Date
      2009-05-31
  • [Presentation] The genus Ostreopsis in the coastal regions in Tosa Bay, southern part of Japan2008

    • Author(s)
      Masao Adachi, Takuji Ikegami, Haruo Yamaguchi
    • Organizer
      The 13th International Conference on Harmful Algae, Poster Presentation
    • Place of Presentation
      The Hong Kong Disneyland Hotel, Hong Kong, China
    • Year and Date
      20081103-07
  • [Presentation] Stable nuclear transformation of the diatom Chaetoceros sp.2008

    • Author(s)
      Arisa Miyagawa, Haruo Yamaguchi and Masao Adachi
    • Organizer
      The 8th Asia-Pacific Marine Biotechnology Conference, Poster Presentation
    • Place of Presentation
      Busan, Korea
    • Year and Date
      20081013-15
  • [Patent(Industrial Property Rights)] 海産藻類を形質転換するために用いられる新規プロモーター2009

    • Inventor(s)
      足立真佐雄、長崎慶三、外丸裕司
    • Industrial Property Rights Holder
      国立大学法人高知大学、独立行政法人水産総合研究センター
    • Industrial Property Number
      特願2009-013577
    • Filing Date
      2009-01-23

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Published: 2010-06-10   Modified: 2016-04-21  

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