Analysis of the mechanism of sex determination and gonadal sexual differentiation of the pufferfish, Takifugu rubripes

2009 Fiscal Year Final Research Report

• Project/Area Number: 19580207
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kyushu University
• Principal Investigator: AKIHIKO Yamaguchi Kyushu University, 農学研究院, 助教 (10332842)
• Co-Investigator(Kenkyū-buntansha): MATSUYAMA Michya 九州大学, 農学研究院, 教授 (00183955)
• Project Period (FY): 2007 – 2009
• Keywords: 内分泌 / 配偶子形成 / 環境要因 / ステロイドホルモン

Research Abstract

In order to assess the involvement of aromatase and sex steroids in gonadal differntiation, an aromatase inhibitor (AI) was administered to developing pufferfish (torafugu) till the 100^<th> day after hatch. AI inhibits ovarian cavity formation, and then irreversible sex change (female to male) was observed. High temperature treatment, which is used for sex reversal of many fishes, induces gonadal degeneration in the pufferfish. We also show that aromatase expression can be regulated by gonadotropin using our newly developed in vitro gonad culture system.

Research Products

• [Journal Article] Estradiol-17βtreatment induces intersexual gonadal development in the pufferfish, Takifugu rubripes. (2009)
  • Author(s): Lee, K-H, Yamaguchi, A, Rashid, H, Kadomura, K, Yasumoto, S., Matsuyama, M
  • Journal Title: Zool. Sci. 26 Pages: 639-645
• [Journal Article] Germcell degeneration in high-temperature treated pufferfish, Takifugu rubripes. (2009)
  • Author(s): Lee, K-H, Yamaguchi, A, Bashid-H, Kadomura, K, Yasumoto, S., Matsuyama, M
  • Journal Title: Sex. Dev. 3 Pages: 225-232
• [Journal Article] 全オス化は可能か?トラフグの性決定・性分化研究 (2008)
  • Author(s): 山日明彦
  • Journal Title: 月刊養殖 7月号 Pages: 14-18
• [Journal Article] (Takifugu rubripes) sexual differentiation: CYP19 regulation and aromatase inhibitor induced testicular development. (2007)
  • Author(s): Fiashid, H, Kitano H, Lee, KH, Nii, S., Shigematsu, T., Kadomura, K, Yamaguchi, A, Matsuyama, M
  • Journal Title: Sex. Dev. 1 Pages: 311-322
• [Presentation] Estradiol-17βtreatment induces ovarian development in testis of pufferfish, Takifugu rubripes. (2008)
  • Author(s): Lee, K-H, Yamaguchi, A, Rashid, H, Kitano, H, Nii, S., Sbigematsu, T., Kadomura, K, Yasumoto, S., Matsuyama, M.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: 横浜
  • Year and Date: 2008-10-23
• [Presentation] トラフグ精巣に存在する2種類のbrain-typeアロマターゼmRNAの発現と分布。 (2008)
  • Author(s): Rashid, H, Lee K-H, Matsuyama, M., Yamaguchi, A
  • Organizer: 第79回日本動物学会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-07
• [Presentation] Modulation of aromatase activity results in masculinization of Japanese tiger puffer Takifugu rubripes. (2008)
  • Author(s): Rashid H, Yamaguchi A, Matsuyama M.
  • Organizer: 15th International Gongress on Biotechnology in Animal Reproduction
  • Place of Presentation: Mymensingh, Bangl adesh
  • Year and Date: 2008-08-06
• [Presentation] Effects of exogenous factors on gonadal sex differertiation and development of fugu Takifugu rubripes. (2008)
  • Author(s): Rashid H, Lee K-H, Kadomura K, Yamaguchi, A, Matsuyama, M.
  • Organizer: World Aquacul ture 2008
  • Place of Presentation: 釡山
  • Year and Date: 2008-05-19
• [Presentation] 未成熟期トラフグの脳下垂体における生殖腺刺激ホルモン(GtH)mRNAの発現解析。 (2008)
  • Author(s): 重松亨・李敬勲・新居早也佳・Harunur Rashid・山口明彦・松山倫也
  • Organizer: 平成19年度日本水産学会九州支部大会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2008-01-26
• [Presentation] トラフグ精巣セルトリ細胞の培養系の開発 (2008)
  • Author(s): 新居早也佳・李敬勲・山日明彦・松山倫也
  • Organizer: 平成19年度日本水産学会九州支部大会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2008-01-26
• [Presentation] Production of all male population of torafugu (Takifugu rubripes) using an aromatase inhibitor (2007)
  • Author(s): Rashid, H, Kitano, H, Lee, K, H, Nii, S., Shigematsu, T., Yamaguchi, A, Matsuyama, M.
  • Organizer: fadrozole The 4th International Joint Symposium betveen Japan and Korea
  • Place of Presentation: 鳥取大学
  • Year and Date: 20071100
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/asweb/suil/lmb.html