A study of high weight molecular kininogen domain 5 derived peptide : Inhibition of metastasis and vascularization, and induction of apoptosis

2008 Fiscal Year Final Research Report

• Project/Area Number: 19590304
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Shiga University of Medical Science
• Principal Investigator: OHKUBO Iwao Shiga University of Medical Science, 医学部, 教授 (80152073)
• Co-Investigator(Kenkyū-buntansha): UEYAMA Hisao 滋賀医科大学, 医学部, 准教授 (30127013) ; MAEDA Toshinaga 滋賀医科大学, 医学部, 助教 (20250342) ; TAKEUCHI Keisuke 滋賀医科大学, 医学部, 助教 (40432306)
• Project Period (FY): 2007 – 2008
• Keywords: 分子腫瘍学

Research Abstract

ヒト血液凝固内因系・高分子キニノーゲンの分解産物であるドメイン5(D5_H)の持つ細胞の接着阻害, 走化阻害, 浸潤阻害とその阻害活性機構, 内皮細胞管腔形成阻害, アポトーシス誘導活性を検討した。(1)細胞の種類と誘因タンパク質(細胞外基質タンパク質)の種類にかかわらず, D5_HおよびD5_H由来ペプチドによる細胞の接着阻害, 走化阻害, 浸潤阻害が見られた。(2)阻害活性を発揮するD5_H の領域に結合する細胞膜画分を同定した。 (3) 内皮細胞管腔形成阻害活性を持つと思われる領域を同定した。(4)アポトーシス誘導活性は細胞接着阻害実験後の細胞において認められなかった。(5)抗体を用いた細胞染色法によるERK1/(2)とAktのリン酸化の度合の変化は, 細胞接着阻害実験後の細胞では見られなかった。

Research Products

• [Journal Article] Novel KCNE3 mutation reducesrepolarizing potassium current and associated with long QT syndrome (2009)
  • Author(s): Ohno S, Toyoda F, Zankov DP, Yoshida H, Makiyama T, Tsuji K, Honda T, Obayashi K, Ueyama H, Shimizu W, Miyamoto Y,Kamakura S, Matsuura H, Kita T, Horie M
  • Journal Title: Hum. Mutat. 30(4) Pages: 557-563
  • Peer Reviewed
• [Journal Article] Shedding of gACE from residual body membrane of rat sperm (2009)
  • Author(s): Takeuchi K, Sakaue T, Yamamoto Y, Nishi K, Ohkubo I
  • Journal Title: Leg. Med.(Tokyo) 11 Pages: S309-S310
  • Peer Reviewed
• [Journal Article] Legumain/asparaginyl endopeptidase controls extracellular matrix remodeling through the degradation of fibronectin in mouse renal proximaltubular cells (2007)
  • Author(s): Morita Y, Araki H, Sugimoto T, TakeuchiK, Yamane T, Maeda T, Yamamoto Y, NishiK, Asano M, Shirahama-Noda K, NishimuraM, Uzu T, Hara-Nishimura I, Koya D,Kashiwagi A, Ohkubo I
  • Journal Title: FEBS Lett 581 Pages: 1417-1424
  • Peer Reviewed
• [Journal Article] Porcine germinal angiotensin I-converting enzyme : Isolation andcharacterization and molecular cloning (2007)
  • Author(s): Takeuchi K, Araki H, Sakaue T, Yamamoto Y, Fujiwara M, Nishi K, Ohkubo I
  • Journal Title: Comp. Biochem. Physiol. part B 146 Pages: 215-226
  • Peer Reviewed
• [Presentation] Identification of actinin-4 as a binding protein for domain 5 of high molecular weight kininogen on MG-63 (2008)
  • Author(s): Tunetoshi Hato, Toshinaga Maeda, Susumu Uchiyama, Daisuke Nou, Keisuke Takeuchi,Osamu Ogikubo, Iwao Ohkubo and Takanobu Otsuka
  • Organizer: The 23^<rd> Annual Research Meeting of theJapanese Orthopaedic Association
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-10-24
• [Presentation] ヒトキニノーゲンdomain 5由来ペプチドによるMG-63細胞における遺伝子発現変化 (2007)
  • Author(s): 山根拓也, 渡辺一史, 杉本直之, 蜂須麗, 竹内圭介, 前田利長, 大久保岩男
  • Organizer: BMB2007
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14