The role of AP2β, the molecule which leads to adipocyte hypertrophy, in the metabolic syndrome

2009 Fiscal Year Final Research Report

• Project/Area Number: 19591045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Shiga University of Medical Science
• Principal Investigator: UGI Satoshi Shiga University of Medical Science, 医学部, 助教 (20378483)
• Co-Investigator(Kenkyū-buntansha): MAEDA Shiro 独立行政法人理化学研究所, 遺伝子多型センター, チームリーダー (50314159)
• Project Period (FY): 2007 – 2009
• Keywords: メタボリックシンドローム / 内臓脂肪 / アジポカイン / AP2β

Research Abstract

To determine the potential role of TFAP2B in the regulation of expression of adiponectin, leptin and interleukin-6 (IL-6) in vivo, we quantified the mRNA expression levels of these adipokines and TFAP2B in visceral (omental) and abdominal subcutaneous adipose tissues of 66 individuals and studied their correlations. We found that TFAP2B mRNA correlated negatively with adiponectin expression in the subcutaneous adipose tissue. Furthermore, TFAP2B mRNA expression correlated negatively with leptin and positively with IL-6 expression in both subcutaneous and omental adipose tissues. These relationships are consistent with our in vitro observations and indicate that TFAP2B seems to regulate the expression of various adipokines in vivo.

Research Products

• [Journal Article] Transcription factor AP-2beta: a negative regulator of IRS-1 gene expression. (2010)
  • Author(s): Meng X, Kondo M, Morino K, Fuke T, Obata T, Yoshizaki T, Ugi S, Nishio Y, Maeda S, Araki E, Kashiwagi A, Maegawa H.
  • Journal Title: Biochem Biophys Res Commun. 392(4) Pages: 526-32
  • Peer Reviewed
• [Journal Article] Relation of the expression of transcriptional factor TFAP2B to that of adipokines in subcutaneous and omental adipose tissues. (2009)
  • Author(s): Ugi S, Nishio Y, Yamamoto H, Ikeda K, Kobayashi M, Tsukada S, Kondo M, Morino K, Obata T, Yoshizaki T, Uzu T, Naitoh H, Tani T, Maeda S, Kashiwagi A, Maegawa H.
  • Journal Title: Osesity (in press)
  • Peer Reviewed
• [Journal Article] Membrane localization of protein-tyrosine phosphatase 1B is essential for its activation of sterol regulatory element-binding protein-1 gene expression and consequent hypertriglyceridaemia. (2009)
  • Author(s): Ugi S, Shi K, Nishio Y, Shimizu S, Guo B, Sekine O, Ikeda K, Egawa K, Yoshizaki T, Nagai Y, Koya D, Takada T, Torii R, Kimura H, Kashiwagi A, Maegawa H.
  • Journal Title: J Biochem. 146(4) Pages: 541-7
  • Peer Reviewed
• [Journal Article] SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene. (2009)
  • Author(s): Omura Y, Nishio Y, Takemoto T, Ikeuchi C, Sekine O, Morino K, Maeno Y, Obata T, Ugi S, Maegawa H, Kimura H, Kashiwagi A.
  • Journal Title: BMB Rep. 42(4) Pages: 232-7
  • Peer Reviewed
• [Journal Article] Transcription factor AP-2beta: a positive regulator of MCP-1 gene expression. (2009)
  • Author(s): Kondo M, Maegawa H, Obata T, Ugi S, Ikeda K, Morino K, Nakai Y, Nishio Y, Maeda S, Kashiwagi A.
  • Journal Title: Endocrinology. 150(4) Pages: 1654-61
  • Peer Reviewed
• [Journal Article] Membrane localization of protein-tyrosine phosphatase 1B is essential for its activation of sterol regulatory element-binding protein-1 gene expression. (2007)
  • Author(s): Shi K, Ugi S, Shimizu S, Sekine O, Ikeda K, Egawa K, Yoshizaki T, Nagai Y, Nishio Y, Takada T, Torii R, Kimura H, Kashiwagi A, Maegawa H.
  • Journal Title: Biochem Biophys Res Commun. 363(3) Pages: 626-32
  • Peer Reviewed
• [Journal Article] Short-term low-dosage pioglitazone treatment improves vascular dysfunction in patients with type 2 diabetes. (2007)
  • Author(s): Maegawa H, Nishio Y, Nakao K, Ugi S, Maeda K, Uzu T, Kashiwagi A.
  • Journal Title: Endocr J. 54(4) Pages: 613-8
  • Peer Reviewed
• [Journal Article] Effects of a Fish-Based Diet on the Serum Adiponectin Concentration in Young, Non-Obese, Healthy Japanese Subjects.
  • Author(s): Kondo K, Morino K, Nishio Y, Kondo M, Fuke T, Ugi S, Iwakawa H, Kashiwagi A, Maegawa H.
  • Journal Title: J Atheroscler Thromb. (in press)
  • Peer Reviewed
• [Journal Article] Transcription factor AP-2beta inhibits expression and secretion of leptin, an insulin-sensitizing hormone, in 3T3-L1 adipocytes.
  • Author(s): Fuke T, Yoshizaki T, Kondo M, Morino K, Obata T, Ugi S, Nishio Y, Maeda S, Kashiwagi A, Maegawa H.
  • Journal Title: Int J Obes (Lond) (in press)
  • Peer Reviewed
• [Presentation] 肥満2型糖尿病発症候補遺伝子AP-2βは (2009)
  • Author(s): 近藤基之, 前川聡, 小畑利之, 福家智也, 森野勝太郎, 吉崎健, 卯木智, 西尾善彦, 前田士郎, 柏木厚典
  • Organizer: PKCμによる活性調節を受け炎症性サイトカインの分泌を制御する
  • Place of Presentation: 日本糖尿病学会総会
  • Year and Date: 20090000
• [Presentation] 肥満2型糖尿病発症候補遺伝子は (2009)
  • Author(s): 福家智也, 前川聡, 近藤基之, 森野勝太郎, 吉崎健, 小畑利之, 卯木智, 西尾善彦, 前田士郎, 柏木厚典
  • Organizer: レプチン発現を負に制御する
  • Place of Presentation: 日本糖尿病学会総会
  • Year and Date: 20090000
• [Presentation] 肥満2型糖尿病発症候補遺伝子は (2008)
  • Author(s): 福家智也, 前川聡, 近藤基之, 池田和弘, 森野勝太郎, 小畑利之, 卯木智, 西尾善彦, 前田士郎, 柏木厚典
  • Organizer: レプチン遺伝子発現を負に制御する
  • Place of Presentation: 日本糖尿病学会総会
  • Year and Date: 20080000
• [Presentation] 肥満2型糖尿病発症候補遺伝子は (2008)
  • Author(s): 近藤基之, 前川聡, 福家智也, 池田和弘, 森野勝太郎, 小畑利之, 卯木智, 西尾善彦, 前田士郎, 柏木厚典
  • Organizer: MCP-1分泌をプロモーターレベルから正に制御する
  • Place of Presentation: 日本糖尿病学会総会
  • Year and Date: 20080000
• [Presentation] The Transcrption Factor AP-2beta thecandidate gene for causing metabolic syndrome (2008)
  • Author(s): 卯木智、前川聡、柏木厚典
  • Organizer: Diabetes Leading Edge Conference
  • Year and Date: 20080000
• [Presentation] SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene (2008)
  • Author(s): Omura Y, Nishio Y, Takemoto T, Ikeuchi C, Sekine O, Morino K, Maeno Y, Obata T, Ugi S, Maegawa H, Kimura H, Kashiwagi A.
  • Organizer: The 68th annual meeting of American Diabetes Association
  • Year and Date: 20080000
• [Presentation] Activation of Transcription factor AP-2beta: mediated by protein kinase C aggravates expression of inflammatory adipokines in differentiated adipocytes. (2008)
  • Author(s): Kondo M, Maegawa H, Morino K, Fuke T, Obata T, Ugi S, Nishio Y, Maeda S, Kashiwagi A.
  • Organizer: The 68th annual meeting of American Diabetes Association
  • Year and Date: 20080000
• [Presentation] 皮下および内臓脂肪組織生検によるアディポサイトカイン調節機構の検討 (2008)
  • Author(s): 卯木智, 前川聡, 柏木厚典
  • Place of Presentation: 日本肥満学会
  • Year and Date: 20080000
• [Presentation] 肥満2型糖尿病発症関連遺伝子はMCP-1分泌を正に制御する (2007)
  • Author(s): 近藤基之、前川聡、池田和弘、福家智也、森野勝太郎、小畑利之、卯木智、西尾善彦、前田士郎、柏木厚典
  • Organizer: 分子糖尿病シンポジウム
  • Year and Date: 20070000