2009 Fiscal Year Final Research Report
Development of new-generation gene vectors by a control of intracellular trafficking and dispersion based in a real-time imaging of DNA
| Project/Area Number |
19680025
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
AKITA Hidetaka Hokkaido University, 大学院・薬学研究院, 助教 (80344472)
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| Project Period (FY) |
2007 – 2009
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| Keywords | リアルタイムイメージング / 細胞内動態制御 / 遺伝子デリバリー |
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| Research Abstract |
In this project, intracellular trafficking of nano-carrier was controlled from the point of view of intracellular dynamics (i.e. vesicular transport and/or microtubule-dependent transport), dispersity and homogeneity to improve the transfection activity. These technologies will be valuable as used in DNA vaccine and liver-targeting gene carriers. Furthermore, novel peptide was discovered, which confer the activity to pass through the cell monolayer via transcytosis pathway. This device will be useful to overcome the vesicular barriers which limits the delivery to tissue parenchyma.
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[Journal Article] Multi-layered nano particles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process.2009
Author(s)
H.Akita, A.Kudo, A.Minoura, M.Yamaguchi, I.A Khalil, R.Moriguchi, T.Masuda, R.Danev, K.Nagayama, K.Kogure, H.Harashima
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Journal Title
Biomaterials 30
Pages: 2940-2949
Peer Reviewed
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[Journal Article] Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancer vaccines
Author(s)
H.Akita, K.Kogure, R.Moriguchi, Y.Nakamura, T.Higashi, T.Nakamura, S.Serada, M.Fujimoto, T.Naka, S.Futaki, H.Harashima
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Journal Title
Programmed endosomal escape and dissociation (in press)
Peer Reviewed
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